中文摘要：

目的：研究化痰中药对C57黑鼠膝骨关节炎滑膜中COMP基因表达的影响。方法：以7周龄C57黑鼠48只为模型,随机分为4组：空白组（K）、模型组（M）、化痰组（T）、芬必得组（F）。每组12只,雌雄各半。先进行4周跑步造模,再分别予生理盐水、化痰方（牛蒡子汤）、芬必得灌胃,在给药4周后取材,应用Trizol法抽提总RNA,RT-PCR技术半定量检测膝关节滑膜中COMP基因表达,采用天能GIS凝胶图像处理系统分析各组表达灰度值。结果：模型组与空白组相比,滑膜中COMP基因表达明显升高（P〈0.01）,两个治疗组与模型组比较,滑膜中COMP基因表达明显降低（P〈0.05）,化痰组与芬必得组比较,滑膜中COMP基因表达明显降低（P〈0.01）,两者有显著性差异。结论：化痰中药可以下调C57黑鼠膝骨关节炎滑膜中COMP基因的表达水平,可以延缓滑膜的增生和纤维化,减少滑膜分泌炎症介质和软骨降解酶,对骨关节炎有明显的防治作用,化痰中药组明显好于芬必得组。

英文摘要：

Objective：To observe the effect of Diminishing Sputum Recipe on gene expression of cartilage oligomeric matrix protein （COMP） in knee Osteoarhritic synovium of C57 black mice. Methods： Forty-eight C57 black mice at the age of 7 weeks were randomized into normal group, model group, Diminishing Sputum Recipe group, and Febid group, Osteoarhri tis model was established by four-week running. Sodium Chloride, Diminishing Sputum Recip（Arctium Lappa Soup）, Febid were administrated to different groups of mice respectively. Four weeks after administration, total RNA in synovium was extracted by Trizol, and expressions of COMP gene were detected by the means of RT-PCR. Results：The expression of COMP gene was significantly higher in model group than in normal group（P〈0.01） ; the expressions of COMP gene were lower in two treatment groups than in model group（P〈0.05）; The expression of COMP gene was lower in Diminishing Sputum Recip group than in Febid group （P〈0.01）. Conclusion：Diminishing Sputum Recipe may prevent and cure osteoarthritis through regulating the gene expression of COMP in osteoarthric synovium of knee to suspend synovial hyperplasia and fibrosis, to reduce synovium excreting inflammatory matter and cartilaginous decomposing enzyme. The Diminishing Sputum Recip group may be superior to Febid group.