中文摘要：

目的 制备甘氨酰组氨酰赖氨酸-铜（GHK-Cu）脂质体,观察其对脱发模型小鼠毛发生长的影响.方法？利用反向蒸发法制备包含GHK-Cu脂质体.将脱发模型小鼠随机分组,各组分别以0.6 mol·L-1GHK-Cu溶液、0.6 mol·L-1GHK-Cu脂质体溶液和纯化水（对照组）涂擦脱毛区,记录小鼠新生毛发生长时间,观察脱毛区病理及组织形态学变化,Western blotting法分析各组小鼠血管内皮生长因子（VEGF）表达差异.结果GHK-Cu脂质体和GHK-Cu均能促进小鼠毛发生长;与对照组比较,GHK-Cu脂质体和GHK-Cu组小鼠毛囊数目差异有统计学意义.在小鼠毛发完全长出之前,GHK-Cu脂质体和GHK-Cu组小鼠VEGF表达量高于对照组.结论？脂质体既能够有效包裹GHK-Cu,又能保持GHK-Cu对小鼠的促生发功效.GHK-Cu脂质体和GHK-Cu均能增加生发初期小鼠皮肤组织VEGF表达.

英文摘要：

Objective To prepare GHK-Cu liposome and analyze its effect on hair growth in mouse alopecia model. Methods Reverse evaporation method was used for GHK-Cu liposome preparation. The alopecia model was established by spreading 6% of Na2 S solution on the back of KM mice. The mice were divided into three groups randomly and treated with 0.6 mol · L-1 of GHK-Cu,0.6 mol · L-1 of GHK-Cu liposome,or distilled water once daily,respectively. The new hair growth status was recorded every day. Paraffin sections were obtained from treated skin tissues post treatment, and the pathological and histological changes were evaluated. Western blotting was carried out to detect VEGF expression in the three groups. Results Both of GHK-Cu liposome and GHK-Cu could promote hair growth in the mouse alopecia model. The number of hair follicles in the two groups was significantly different from that of the control group. VEGF expression in GHK-Cu and GHK-Cu liposome groups was higher than that in the control group before the hair grew completely. Conclusion Liposome can effectively encapsulate GHK- Cu, and maitain the function of GHK-Cu in promoting hair grow. Both GHK-Cu liposome and GHK-Cu can increase VEGF expression in mouse skin at early stage of hair growth.