中文摘要：

目的：探讨神经降压素（neurotensin,NTS）与肝细胞肝癌（hepatocellular carcinoma,HCC）生长和侵袭性的关系。方法：随机选取天津医科大学肿瘤医院100例经部分肝切除术治疗的HCC患者的临床病理资料,并分析NTS、神经降压素受体1（neurotensin receptor 1,NTR1）与临床病理指标的相关性。以Hep3B细胞为基础,通过基因转染技术和RNA干扰技术构建不同NTR1表达水平的HCC细胞系。利用BrdU增殖实验、Annexin V凋亡实验、划痕修复实验、Transwell侵袭实验来观察不同NTS处理和不同NTR1表达水平的细胞在增殖、凋亡、迁移、侵袭上的差异。结果：NTS、NTR1表达与HCC患者包膜完整性和门静脉癌栓浸润等转移指标密切相关。外源性NTS刺激和高表达NTR1对Hep3B细胞的增殖与凋亡无影响。Hep3BNTR1hi细胞的划痕修复率和侵袭细胞数均显著高于Hep3Bwt细胞,Hep3BNTR1-细胞的划痕修复率和侵袭细胞数均显著低于Hep3Bwt细胞,同样NTS处理的Hep3Bwt、Hep3BNTR1hi细胞的划痕修复率和侵袭细胞数均高于对照组。结论：HCC组织中NTS、NTR1高表达与肝癌高侵袭性相关,外源性NTS刺激和高表达NTR1不影响HCC的增殖凋亡,但会增强其侵袭性。

英文摘要：

Objective： To study the effects of neurotensin（NTS） on the growth and invasion of hepatocellular carcinoma（HCC）. Methods： The clinicopathological characteristics of 100 patients with HCC were analyzed. The correlations between NTS and neurotensin receptor 1（NTR1） expression and clinicopathological parameters were identified. Hep3B hepatoma cells with different levels of NTR1 were established via gene transfection and si RNA interference. Brd U proliferation assay, Annexin V apoptosis assay, scratch repair experiments, and Transwell invasion assay were used to compare the functional alterations of hepatoma cells upon different NTS stimulation. Results： NTS/NTR1 expression in tissues was significantly correlated with incomplete envelope and portal vein invasion of HCC patients. Exogenous NTS stimulation and NTR1 over-expression do not affect proliferation and apoptosis of HCC cell lines. The wound closure rate and invasion cell number of Hep3BNTR1hi were significantly higher than those of Hep3Bwt; in addition, the wound closure rate and invasion cell number of Hep3BNTR1- are lower than those of Hep3 Bwt. In parallel, NTS-treated Hep3Bwt and Hep3BNTR1hi cells have higher wound closure rates and invasion cell number than NTS untreated cells. Conclusion： High NTS/NTR1 expression correlated with aggressive phenotype of HCC. Exogenous NTS stimulation and NTR1 over-expression do not affect proliferation and apoptosis of HCC cell lines, but can improve invasion.