以聚乙烯醇(PVA)和海藻酸钠(SA)为载体材料,制备PVA凝胶小球对所筛选的真菌进行固定,对PVA小球的机械稳定性、化学稳定性、溶胀性、渗透性、微观结构以及细胞毒性进行测试,并以固定化真菌对不同合成染料废水和工厂印染废水进行脱色研究.结果表明:PVA小球具有较高的机械和化学稳定性,渗透性良好,细胞毒性较低;菌株对直接湖蓝5B、直接耐晒大红4BS为吸附脱色,5 d后脱色率分别达到92%和59%,而对酸性大红3R和工厂印染废水为降解脱色,5 d后脱色率均达到90%左右;固定化菌株经4次重复利用后,对酸性大红3R的脱色率仍超过92%,显示出良好的重复利用性.
The fungus screened from soil was enclosed in the hydrogel beads made from polyvinyl alcohol and sodium alginate. PVA beads were investigated in terms of chemical stability, mechanical stability, swelling ratio, permeability, microstructure and cytotoxieity. The decolorization efficiency of the immobilized fungus on different artificial dyeing wastewater and real dyeing water from plant were investigated. The results revealed that the PVA beads have high mechanical and chemical stability, good permeability, low cytotoxicity. The decolorizations of direct sky blue 5B and direct fast scarlet 4BS were mainly due to the adsorption of dyes by fungus and the decolorization rate of dye reached 92% and 59% after 5 days, respectively. However, the decolorizations of acid red 3R and real dyeing wastewater from plant were caused mainly by the biological degradation and the decolorization rate of dye both reached about 90% after 5 days. Furthermore, immobilized fungus exhibited excellent reusability and its deeolorization rate for acid red 3R reached 92% after four repeat using.