中文摘要：

糖尿病和许多另外的新陈代谢症候群是仔细与肥胖有关。揭示胖免职的内在的机制，越来越多的研究在 adipocytes 开发期间正在集中于 miRNAs 的功能。以前的研究 haveproved 那出 miR-15a/b 戏在多重生理的过程的重要角色；然而，他们的功能在 adipogenesisremain 期间不清楚。揭示这，我们在猪的 pre-adipocyte 在 adipogenesis 期间检测了 miR-15a/b 的表示侧面，并且发现那他们层次在 adipocyte 区别的早阶段增加了并且在白天 4 .Moreover 以后落下的表示，在猪的 pre-adipocytes 的 miR-15a/b 的在表示上支持了 adipocyte 区别， miR-15a/b 的类脂化合物 accumulation.Target 基因被预言并且检验，它表明 Forkhead 盒子蛋白质 O1 ( FoxO1 )是目标 geneof miR-15a/b 。表示水平由 miR-15a/b 在表示上引起了的 FoxO1 的抑制在 adipogenesis.Thus 上有积极效果，我们断定 miR-15a/b 经由镇压在猪的 pre-adipocyte 支持 adipogenesis FoxO1。

英文摘要：

Diabetes and many other metabolism syndromes are closely related to obesity. To reveal the underlying mechan ism of fat deposition, an increasing number of studies are focusing on the functions of miRNAs during adipocytes de velopment. Previous studies have proved that miR15a/b play important roles in multiple physiological processes; however, their functions during adipogenesis remain un clear. To reveal this, we detected the expression profiles of miR15a/b during adipogenesis in porcine preadipocyte, and found that their expression levels increased in the early stage of adipoeyte differentiation and dropped after day 4. Moreover, overexpression of miR15a/b in porcine pre adipocytes promoted adipocyte differentiation and lipid accumulation. Target genes of miR15a/b were predicted and examined, which revealed that Forkhead box protein O1 （FoxO1） is the target gene of miR15a/b. The inhibition of FoxO1 expression level caused by miR15a/b over expression had a positive effect on adipogenesis. Thus, we conclude that miR15a/b promote adipogenesis in porcine preadipocyte via repressing FoxO1.