中文摘要：

肌动蛋白细胞骨架是橡胶树乳管伤口堵塞物的成分之一。为了研究乳管伤口末端与肌动蛋白互作的蛋白．成功构建了ACTIN基因的原核表达载体。在37℃条件下，经0．1mmol／LIPTG诱导2h，在EcoilBL21（DE3）中大量表达重组蛋白。该重组蛋白主要以包涵体形式存在，分子量41．7ku。通过Ni—NTA亲和层析柱纯化了该重组蛋白，并用抗HIS标签的鼠单克隆抗体对纯化蛋白进行鉴定。本研究为揭示乳管伤口末端堵塞物形成机制打下了良好基础。

英文摘要：

ACTIN, a component of cytoskeleton, is one of the constituents of the plugs at the end of the severed laticifers. In order to study the interaction of ACTIN with proteins in the plugs, the A CTIN gene prokaryotic expression vector was successfully constructed. Recombinant protein was expressed in abundance in the E.coil BL21 （DE3） after being induced by 0.1 mmol/L IPTG for 2 hours at 37 ℃. The recombinant protein with a molecular mass of about 41.7 ku was mainly present as inclusion body. It was further purified by Ni-NTA affinity chromatography and identified by western-blot with mouse anti-HIS tagged monoclonal antibody. This study laid a good foundation of revealing the mechanism for plug formation at the end of severed laticifers.