中文摘要：

目的观察针刺腧穴＂降脂方＂对高脂血症大鼠羟甲基戊二酰辅酶还原酶（HMG-Co A）、低密度脂蛋白受体（LDL-R）、胆固醇7α-羟化酶（CYP7A1）的影响,进而探讨针刺＂降脂方＂逆转高脂血症的初步机制。方法 44只大鼠用随机数表法随机分为正常组17只、模型组27只,分别给予普通饲料、高脂饲料喂养。3周后各组随机选7只经腹主动脉采血,观察总胆固醇（TC）;三酸甘油（TG）;低密度脂蛋白-胆固醇（LDL-C）及高密度脂蛋白胆固醇（HDL-C）含量变化。再将模型组随机数表法随机分为对照组和降脂方组进行干预。4周后观察各组大鼠TC、TG、LDL-C、HDL-C的含量、用Real-time PCR方法进行测定肝脏中的HMG-Co A、LDL-R和CYP7A1 m RNA表达。采用SPSS13.0统计软件数据分析,数据资料为计量资料,且呈正态分布,以均数±标准差（χ±s）表示,两组间比较采用成组设计资料的t检验,3组间比较采用单因素方差分析,以P〈0.05为差异有统计学意义。结果喂养3周后,与正常组比较,大鼠血清TC、TG、LDL-C升高,HDL-C降低（t=4.09、3.32、5.22、14.57,P〈0.01或P〈0.05）,模型组为高血脂状态。分组后模型组与正常组比较：肝脏中HMG-Co A表达显著高于正常组,LDL-R、CYP7A1水平显著低于正常组（t=54.52、11.78、6.02,均P〈0.01）;干预4周后,腧穴＂降脂方＂组与模型组比较：大鼠血清TC、TG及LDL-C降低,HDL-C升高（t=2.67、2.77、2.76、3.74,均P〈0.05）;肝脏中HMG-Co A表达降低显著,LDL-R、CYP7A1水平明显升高（t=34.99、4.18、12.95,均P〈0.01）。结论腧穴＂降脂方＂对大鼠高脂血症具有较好的防治作用,其作用机制与降低血脂、逆转肝脏组织中与脂代谢相关酶的表达有关。

英文摘要：

Objective To observe the effect of electric acupuncture acupoint recipes＂lipidlowering＂on hydroxymethylglutaryl-coenzyme A（HMG-Co A）, low density lipoprotein receptor（LDL-R） and cholesterol 7 alpha hydroxylase（CYP7A1） in fatty liver rat model with hyperlipidemia, and the mechanisms thereof. Methods Forty-four SD rats were randomly divided into two groups： control group（n=17） and model group（n=27）. The rats of control group were fed standard diet and rats in model group were given high fat diet. Blood samples were collected from 7 rats of each group after 3-week treatment. Changes of total cholesterol（TC）, triglyceride（TG）, low density lipoprotein cholesterol（LDL-C） and high density lipoprotein cholesterol（HDL-C） were detected and compared between two groups. The model group was randomly subdivided into control group and lipid-lowering group. Changes of TC, TG, LDL-C andHDL-C were observed after 4-week treatment in four groups. RT-PCR method was used to detect the values of HMG-Co A, LDL-R and CYP7A1 m RNA expression in liver tissue. SPSS13.0 statistical software was used for data analysis. The t-test was used in the comparison between the two groups, and single factor analysis of variance was used for data analyses of three groups. Statistically significance was considered when P0.05. Results After three weeks, compared with control group, serum levels of TC, TG and LDL-C were significantly increased in model group but HDL-C were significantly decreased（t=4.09, 3.32, 5.22 and 14.57,P0.01 or P0.05）. The model rats were hyperlipidemia status. The expression of HMG-Co A was significantly higher in model group than that of control group（P0.01）, and expression levels of LDL-R and CYP7A1 were significantly lower in model group than those of control group（t=54.52, 11.78 and 6.02, P0.01）. After 4-week acupuncture treatment, the serum levels of TC, TG and LDL-C were significantly decreased, the level of HDL-C was significantly increased, in lipid-lowering group than th