中文摘要：

克隆水稻尿苷二磷酸葡萄糖焦磷酸化酶基因（OsUgp1）的启动子,并与大肠杆菌β-葡萄糖苷酸酶（β-glucu-ron idase,GUS）报告基因连接后转化水稻,采用GUS组织化学染色检测启动子驱动GUS基因在水稻转化植株中的表达情况,发现在水稻的根、茎、叶、内外稃、子房、柱头等组织,以及减数分裂时期的花药及不同发育时期的胚乳和胚中均有表达.通过与水稻中另一同源基因（OsUgp2）的启动子GUS融合基因在种子发育过程的表达进行比较,发现两者有明显的差异：OsUgp1基因在开花后5-20 d的胚乳和胚中表达量都比较高,而OsUgp2基因几乎不表达.进一步通过对OsUgp1基因和OsUgp2基因启动子区有关胚乳特异表达相关顺式调控元件在数量和分布位置上的差异分析,探讨了该2个同源基因在种子发育中趋异表达可能的分子机制.

英文摘要：

Promoter of one UDP-glucose pyrophosphorylase gene（OsUgp1） was cloned,fused with β-glucuronidase（GUS） reporter gene and introduced intro rice.Histochemical analysis of GUS activity was used to detect the GUS expression in transgenic rice.The result showed that GUS expressed in root,stem,leave,palea,lemma,ovary,stigma,anther at meiosis stage,developing endosperm and embryo.The expression of OsUgp1 gene in developing seed was compared with that of OsUgp2 gene.An eminent difference between OsUgp1 gene and OsUgp2 gene was found.OsUgp1 gene expressed in endosperm and embryo in the seed 5-20 d after flowering,but OsUgp2 gene almost did not express.The number and location of the cis-element related to endosperm-specific expression were analyzed in the two promoter regions,and a possible molecular mechanism of the divergent expression of two homologous genes was discussed.