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Characterization of four hemocyanin isoforms in Litopenaeus vannamei

ISSN号：0253-505X
期刊名称：《海洋学报：英文版》
时间：0
分类：Q785[生物学—分子生物学] S968.22[农业科学—水产养殖;农业科学—水产科学]
作者机构：[1]School of Life Science, Xiamen University, Xiamen 361005, China, [2]Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China, [3]Zhanjiang Ocean and Fishery Development Research Center, Zhanjiang 524000, China, [4]School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China, [5]Zhanjiang Evergreen South Ocean Science & Technology Company Limited, Zhanjiang 524094, China
相关基金：The National High Technology Research and Development Program（863 Program）of China under contract No.2012AA092205; the Major State Basic Research Development Program（973 Program）of China under contract No.2012CB114403; the China Agriculture Research System-47; the National Natural Science Foundation of China under contract No.31472297 and 31302209; the Scientific Research Foundation of Third Institute of Oceanography,SOA under contract No.2011018

作者： XU lingxiang[1,2], RUAN Lingwei[2], LI Zhen[5], YU Xiaoman[4], LI Sedong[3,5], SHI Hong[2], XU Xun[2]

关键词：凡纳滨对虾, 亚型, 第二外显子, 抗病毒机制, 表征, WSSV, 编码基因, 血蓝蛋白, hemocyanin,SNPs,isoforms,Litopenaeus vannamei,WSSV

中文摘要：

在这研究，编码 hemocyanin 子单元 L 的基因， LvHcL，从 Litopenaeus vannamei 被克隆， genomic 组织被描绘。这基因与许多 SNP 是多样的并且也有至少四 isoforms，当(LvHcL4 ) 他们之一仅仅有二 exons， exon2 被错过时。抄写分析证明 LvHcL 的这些 isoforms 在在 WSSV 抵抗的虾的 WSSV 挑战以后是起来调整的，当抄写在 WSSV 易受影响的虾常常被减少时。hemocyanin 有富有的多型性并且涉及抗病毒的反应，这被建议。这些结果能扩大我们的以前的调查结果并且提供卓见进 hemocyanin 的有免疫力的特征，它将对在无脊椎动物针对抗病毒的机制的进一步的研究有用。

英文摘要：

In this study, the gene encoding hemocyanin subunit L, Lv Hc L, was cloned from Litopenaeus vannamei and the genomic organization was characterized. This gene was diverse with many SNPs and also had at least four isoforms, while one of them（Lv Hc L4） only had two exons and the exon2 was missed. Transcription analysis showed that these isoforms of Lv Hc L were up-regulated after WSSV challenge in WSSV-resistant shrimp, while the transcriptions were decreased constantly in WSSV-susceptible shrimp. It is suggested that the hemocyanin had rich polymorphism and was involved in the antiviral response. These results could extend our previous findings and provide insights into the immune feature of hemocyanin, which would be helpful for further studies aimed at antiviral mechanism in invertebrate.

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