中文摘要：

以地枫皮为试材,采用正交实验研究了无机盐、生长调节素、渗透压等对地枫皮离体保存的影响,建立地枫皮种质资源离体保存技术体系;并通过生长恢复的考察对保存材料进行初步评价。结果表明：无机盐、生长调节剂、渗透压等因素对地枫皮种质资源的离体保存均有显著影响,最终确定地枫皮常温离体保存的最佳保存方法为1/2MS＋蔗糖50g/L＋琼脂4.0g/L＋甘露醇5g/L＋矮壮素（CCC）1.0mg/L,培养条件为光照时间12-14h/d,培养温度25±2℃,光照强度2 000lx,在此条件下保存300d,存活率在50%以上,保存材料生长恢复情况良好。

英文摘要：

Taking Illicium difengpi as test material,to explore a method for the germplasm conservation in vitro of Illicium difengpi,the single buds were used as experimental materials to study the effects of inorganic salts,phytohormone and osmotic pressure,and the growth recovery was evaluated after in vitro conservation.The results showed that the best conservation condition was 1/2MS basal medium containing 50g/L sucrose,4.0g/L agar powder and 5g/L mannite supplemented with 1.0mg/L CCC,in illuminated chamber,12-14hours light/12-10hours dark photoperiod of 2 000 lx light intensity at 25±2℃,the survival rate was 50%after ten month conservation,and most of them could growth well on propagation medium.