中文摘要：

以马来酸酐改性的壳聚糖（MAH-chitosan）和丙烯酸（AA）为单体，采用反相微乳液聚合法制备了AA含量分别为77％（AA-Cs）和29％（Cs-AA）壳聚糖／聚丙烯酸复合纳米粒子。TEM结果表明，该复合纳米粒子平均粒径为125—150nm，进而研究了其对牛血红蛋白（Hb）、牛血清白蛋白（BSA）和溶菌酶（Ly）吸附和脱附行为。AA-Cs对各种蛋白的吸附量均较高；而Cs-AA对3种蛋白的吸附则具有一定选择性。AA-Cs对等电点低于脱附pH值的Hb的脱附量较大。Cs—AA粒子在较低pH值（pH=3．4）时对吸附有利，但在高pH值（pH=6．6）进行吸附时，对脱附更为有利。

英文摘要：

In this work, chitosan-acrylic acid （AA） nanoparticles were prepared by inverse microemulsion polymerization technique with maleic anhydride-modified chitosan and acrylic acid as monomers. The AA-Cs （with AA 77%） and Cs-AA （with AA 29%） nanoparticles have been prepared by changing the ratios of chitosan and AA. The results from the TEM graphs showed that the average size of the particles was about 125-150nm. The adsorption behaviors were measured with hemoglobin （Hb）, bovine albumin （BSA） and lysozyme （Ly） as model protein. Results indicated that the AA-Cs microspheres had high adsorption capacity to the three proteins and could be used as protein adsorbent. The Cs-AA microspheres could be used as selective adsorbent of proteins, and found that the adsorption ability was good at low pH value of 3.4 or 4.4, the desorption ability was good when the adsorption was done at high pH value （pH=6.6） buffer solution.