中文摘要：

目的观察活血益气方及其拆方对心肌梗死后大鼠梗死边缘区心肌组织血管新生负性调控因子Spred1及血管新生的影响,探讨活血益气方促血管新生的可能作用机制及配伍规律。方法健康雄性SD大鼠30只,随机分为假手术组6只与手术组24只,手术组行左冠状动脉前降支结扎术制备急性心肌梗死模型,随机分为模型组、活血益气方组、益气方组、活血方组。假手术组只穿线不结扎。连续给予相应药物4周后,处死大鼠,取梗死边缘区心肌组织进行指标检测。采用免疫组织化学法观察微血管密度（microvasculardensity,MVD）、微血管平均直径（mean microvascular diameter,MMVD）;采用实时荧光定量PCR法检测Spred1 mRNA的表达;采用Western Blot法检测Spred1蛋白的表达。结果 （1）模型组MVD高于假手术组,MMVD小于假手术组（P〈0.01）;活血益气方组、益气方组、活血方组MVD高于模型组（P〈0.01或P〈0.05）,其中以活血益气方组最为明显,MMVD大于模型组,仅活血益气方组与之比较具有统计学差异（P〈0.01）;益气方组、活血方组MMVD小于活血益气方组,分别与之比较,均具有统计学差异（P〈0.01）。（2）模型组梗死边缘区心肌组织Spred1 mRNA表达高于假手术组（P〈0.05）;活血益气方组、益气方组、活血方组Spred1mRNA表达高于模型组,差异具有统计学意义（P〈0.01或P〈0.05）;益气方组、活血方组Spred1mRNA表达均高于活血益气方组,仅活血方组与之比较具有统计学差异（P〈0.01）。（3）模型组梗死边缘区心肌组织Spred1表达高于假手术组,两者比较未见统计学差异（P〉0.05）;活血益气方组、益气方组、活血方组Spred1表达均低于模型组,比较差异具有统计学意义（P〈0.05）;益气方组Spred1表达低于活血益气方组,活血方组Spred1表达高于活血益气方组,分别与之比较,均未见统计学差异（P〉0.05）。结论活血益气方及其

英文摘要：

Objective To observe the impact of Huoxue Yiqi prescription on the negative regulation factor Spred1 and angiogenesis of myocardial tissue in the marginal zone of myocardial infarction rats,and to explore the mechanism and compatibility regularity of the compound decoction of Huoxue Yiqi prescription promoting therapeutic neovascularization. Methods Model rats of acute myocardial infarction（ AMI） were established by ligation of left anterior descending coronary artery,and randomly divided into 4groups. Therapeutic groups were treated with Huoxue Yiqi prescription（ HXYQ）,Yiqi prescription（ YQ）,Huoxue prescription（ HX） and model group. Rats of sham group were treated with saline and operated without ligation. Animals were sacrificed and the myocardial infarction border areas were taken as indicators after 4 weeks of treatment. Microvascular density（ MVD） and the mean microvessel diameter（ MMVD）were assessed by immunohistochemistry. The expression of Spred1 mRNA and VEGF mRNA was detected by quantitative real-time PCR（ qRT-PCR）,and Western Blot was used to observe the expression of Spred1. Results（ 1） The MVD in model group was higher than that in sham group（ P 〈0. 01）. Compared with model group,HXYQ,HX and YQ group can improve MVD（ P 〈0. 01 or P 〈0. 05）,and the effect of HXYQ is the most significant. The MMVD in model group was higher than sham group（ P 〈0. 01）. Compared with model group,HXYQ,HX and YQ group could improve MMVD,but only HXYQ group had significant difference（ P 〈0. 01）. The MMVD in HXYQ group was higher than that in the other two therapeutic groups（ P 〈0. 01）.（2） The mRNA level of Spred1 in model group was higher than that in sham group（ P 〈0. 05）. Compared with model group,HXYQ,HX and YQ group could significantly improve the mRNA level of Spred1（ P 〈0. 05）. The mRNA level of Spred1 in HXYQ group was lower than that in HX group or YQ group. However it was only significantly lower than in HX group（ P 〈0. 01）.（3）