中文摘要：

目的：研究鲫鱼卵唾液酸糖肽（Ca—SGPP）对成骨细胞MC3T3-E1骨形成活性及OPG／RANKL基因表达水平的影响。方法：在MC3T3-E1细胞中加入不同浓度的Ca—SGPP，采用M1-r法、试剂盒和茜素红染色法检测Ca—SGPP对MC3T3—E1细胞增殖、分化和矿化的影响；刺用逆转录聚合酶链式反应（1it—PCR）技术检测MC3T3-E1细胞骨形态发生蛋白-2（BMP-2）、骨保护素（OPG）及核因子KB受体活化因子配体（RANKL）基因mRNA表达水平。结果：Ca—SGPP可显著促进MC3T3-E1细胞增殖及ALP、OCN和COLI的分泌，提高MC3T3-E1细胞矿化能力。上调其BMP一2表达水平及OPG／RANKL的比值，且分子质量大的糖肽效果更佳。结论：Ca—SGPP具有促进MC3T3-E1细胞增殖、分化和矿化的能力，其机制可能与OPG／RANKL比值上调有关。

英文摘要：

Objective： To investigate the effects of sialyglycopeptides from the eggs of Carassius auratus（Ca-SGPP）on the proliferation, diferentiation, mineralization and the gene expression of OPG/RANKL in MC3T3-E1 cells. Method：MTT assay, alkaline phosphatase（ALP） activity assay and calcium deposition were determined, the secretion of collagen I（COL I） and osteocalein（OCN） were assayed by enzyme-linked immunosorbent assay（ELISA）, the m RNA expression levels of bone morphogenetic protein 2（BMP-2）, osteoprotegerin（OPG） and receptor activator for nuclear factor-κB ligand（RANKL） were also measured by reverse transcription polymerase chain reaction（RT-PCR）. Results： The Ca-SGPP could effectively promote the proliferation（P〈0.01）, increase ALP,OCN and COL I content, enhance the mineralization ability, improve the expression levels of BMP-2 and OPG/RANKL ratio of MC3T3-E1 cells. Furthermore, the effect of Ca-SGPP of more than 10 ku was much better than that of less than 10 ku. Conclusion： The Ca-SGPP can remarkably promote the osteogenic activity on MC3T3-E1 cells, and its mechanism is probably related to the rise of OPG/RANKL rato.