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中文摘要：
利用实验室构建的微电纯化装置（Microelectro purification device,MEPD）,采用电喷雾质谱正离子模式检测,在2 min之内实现重组人促红细胞生成素（recombinant human erythropoietin,rhEPO）的在线脱盐,同时和传统的C18色谱柱（150 mm×4.6 mm,5μm）梯度洗脱以及超滤管除盐结果进行比较。利用MEPD除盐后,rhEPO特征离子信噪比增加,检测灵敏度明显提高,操作时间大大缩短。本方法简单、快速,可作为质谱分析rhEPO样品的前处理方法。
英文摘要：
Microelectro purification device（MEPD） constructed by our laboratory could obtain online desalting of recombinant human erythropoietin（rhEPO） in 2 min with high efficiency.Electrospray mass spectrometry with positive ion mode was used for the detection.Compared with traditional methods,such as gradient elution by C18-reversed phase liquid chromatography（150 mm×4.6 mm,5 μm） as well as ultra-filtration,MEPD could further enhance the signal to noise ratio of rhEPO and the detection sensitivity.It could also shorten the analysis time dramatically.This simple and rapid method could be a promising pretreatment candidate for the rhEPO analysis of mass spectrometry.
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