中文摘要：

最近，血浆消毒为气压非平衡血浆喷气(APNP ) 在牙齿的社区吸引了增加的注意，它被千赫驾驶搏动的 DC 力量，可以被用于牙齿、口头的疾病。然而，仍然在疑问 APNP 是否能有效地在口头的洞杀死病原的细菌并且不在正常口头的纸巾上生产有害效果，特别在正常 mucosa 上。这研究的目的是在包含细菌的一个单个品种的 biofilms 评估 APNP 的细菌杀死的效果(Porphyromonas gingivalis， P.g ) ，并且在由 APNP 的处理以后的口头的 mucosa 的病理学的变化。P.g 被孵化在 vitro 形成 biofilms，并且样品随机被划分成三个组：组织 A (空白的控制) ；biofilms 被 APNP 在对待的组 B (设备的背景：10 kHz， 1600 ns 和 8 kV ) ；组织没有血浆的点火， biofilms 仅仅在暴露于一口煤气的喷气的 C。每个组有三件样品，每件样品为多达 5 min 被处理。biofilms 然后荧光灯地在扫描共焦的显微镜的激光下面被染色，观察并且拍摄。在动物实验，六只雄的日本白兔子随机被划分成二个组(在每个组的 n=3 ) 以不同处理以后的时间(1 天的组和 5 天的组) 。左边的口腔的 mucosa 和舌头的腹的表面的 mucosa 被 APNP 在每只兔子以象细菌的 biofilm 实验的一样的方法为 10 min 对待，并且另外的方面的相应 mucosa 用作正常控制。口头的 mucosa 的临床的表明被遵守并且记录每天。兔子在 APNP 处理以后是牺牲的或五天。口头的 mucosa 被收获并且准备到 haematoxylin 和染色曙红的节。临床的观察和组织病理学说的分数被用来估计 mucosal 变化。结果证明明显的 P.g biofilms 在组 A 在细菌的 10 天，和大多数被形成， C 在扫描共焦的显微镜的激光下面是活着的，但是在组 B 的细菌是几乎所有死者。在动物实验，没有溃疡， anabrosis 和口头的 mucositis 在两个被发现 1 天、 5 天的组。分别地，平均粘膜激怒索引在 1 天、 5 天的组是 0.

英文摘要：

Recently, plasma sterilization has attracted increasing attention in dental community ibr the atmospheric pressure non-equilibrium plasma jet （APNPs）, which is driven by a kilohertz pulsed DC power, may be applied to the dental and oral diseases. However, it is still in doubt whether APNPs can effectively kill pathogenic bacteria in the oral cavity and produce no harmful effects on normal oral tissues, especially on normal mucosa. The aim of this study was to evaluate the bacterial-killing effect of APNPs in the biofilms containing a single breed of bacteria （Porphyromonas gingivalis, Pg.）, and the pathological changes of the oral mucosa after treatment by APNPs. P.g. was incubated to form the biofilms in vitro, and the samples were divided into three groups randomly： group A （blank control）; group B in which the biofilms were treated by APNPs （the setting of the equipment： 10 kHz, 1600 ns and 8 kV）; group C in which the biofilms were exposed only to a gas jet without ignition of the plasma. Each group had three samples and each sample was processed for up to 5 min. The biofilms were then fluorescently stained, observed and photographed under a laser scanning confocal microscope. In the animal experiment, six male Japanese white rabbits were divided into two groups randomly （n=3 in each group） in terms of the different post-treatment time （1-day group and 5-day group）. The buccal mucosa of the left side and the mucosa of the ventral surface of the tongue were treated by APNPs for 10 min in the same way as the bacterial biofilm experiment in each rabbit, and the corresponding mucosa of the other sides served as normal control. The clinical manifestations of the oral mucosa were observed and recorded every day. The rabbits were sacrificed one or five day（s） after APNPs treatment. The oral mucosa were harvested and prepared to haematoxylin and eosin-stained sections. Clinical observation and histopathological scores were used to assess mucosal changes. The results showed the obvious P