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中文摘要：
研究微小卡罗藻的生长曲线及不同生长期藻细胞提取液和去藻上清液溶血活性的大小，通过乙酸乙酯和Bligh—Dyer两种方法提取溶血毒素，利用柱层析和薄层层析对溶血毒素成分进行初步的分离与分析。结果表明，藻细胞提取液在4个生长阶段中都具有明显的溶血活性，去藻上清液在平稳期和衰亡期具有明显的溶血活性，延滞期及对数生长期溶血活性很低，表明微小卡罗藻的溶血物质是其自身正常生理代谢产物，且在细胞老化过程中可以释放至水体环境中。两种提取方法都可以将溶血毒素提取出来，进一步的色谱分离和生物活性检测发现微小卡罗藻主要含有3种不同性质的溶血毒素，其溶血作用可能与微小卡罗藻较高含量的多不饱和脂肪酸18：5n3和22：6n3有关。
英文摘要：
Hemolytic activity of the cell lysate and culture medium of Karlodiniurn rnicrum were tested at various algal growth phases, as well as to compare the difference between ethyl acetate and Bligh--Dyer, the major components of the hemolysin were fractionated with column chromatography and thin-layer chromatography. The results showed that the hemolytic activity in cells of K. rnicrum at the four growth phases maintained at a high level, whereas high hemolytic activity was only observed in the stationary and senescent phases in the filtrates the culture media, indicating that the hemolysin of K. micrum are normal physiological metabolites, and can be released into the environment when cells are in aging process. Ethyl acetate extraction and Bligh-Dyer extraction are both feasible to extract the hemolysin. Through chromatographic separation and hemolytic activity assay tracking, we found that there were three major hemolysin fractions in important r Key words： K. micrum, and two polyunsaturated fatty acid, namely 18 ： 5n3 and 22 ： 6n3, may play an ole in hemolysis.
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