中文摘要：

目的证实Shh—Gli信号途径在人神经母细胞瘤细胞株SK—N—AS细胞中的存在，探讨激活该途径对神经母细胞瘤的存活的影响及其作用机制。方法用免疫细胞化学和Western blot检测SK-N—AS细胞株中Ptch-1、Gli-1、Gli-2蛋白的表达，用噻唑蓝（MTT）比色法检测Shh对细胞存活率的影响，用流式细胞术检测细胞凋亡及细胞周期的变化。结果Ptch-1、Gli-1和Gli-2在SK—N—AS细胞株中强阳性表达，Shh使细胞存活率提高了13．9％，该效应具有时间依赖性和剂量依赖性，流式检测显示Shh作用使细胞凋亡率较对照组比较下降31％，周期检测显示Shh应用后，G0／G1期细胞百分比，从对照组61．81％下降至47．29％，S期从16．55％上升至45．66％，以上差异有统计学意义（P〈0．05）。结论激活Shh—Gli信号可减少SK—N—AS细胞的凋亡，加速其进入增殖周期，促进神经母细胞瘤的存活。

英文摘要：

Objective To prove the existence of the Shh-Gli signaling pathway in the neuroblastoma cell line SK-N-AS, and study the role of the pathway in the neuroblastoma survival by using Shh to stimulate the signaling. Methods Western blot and immunocytochemistry were used to detect the expression of Ptch-1, Gli-1, Gli-2 proteins in the cell line SK-N-AS. Shh,an activated chemical drug was used to treat the SK-N-AS cells at different concentrations and time lengths. MTr was used to examine the survival rate of the ceils, and flow cytometry was applied to assay the apoptosis and cell cycles of the ceils. Results There existed the Shh-Gli pathway in the neuroblastoma cell line SK-N-AS. Shh increased the cell survival rate by 13.9% as compared with control group in a time-and concentration-dependent manner. Shh reduced the apoptosis by 31% ,and impulsed the cell cycle from G1 to S phase. Conclusion The activation of the Shh-Gli pathway prolonged the tumor cell survival by affecting the apoptosis and cell cycle.