中文摘要：

研究多种表面活性剂以及溶剂对4-氯-7-硝基苯-2-氧-1,3-二唑（NBD-Cl）衍生氨基酸荧光性质的影响。考察并优化了NBD-Cl与氨基酸的衍生反应条件以及芯片毛细管电泳-激光诱导荧光的分离分析条件。结果表明：在临界胶束浓度以上,阳离子表面活性剂十六烷基三甲基溴化胺（CTMAB）对精氨酸、谷氨酸、天冬氨酸的NBD-Cl衍生物,非离子表面活性剂十二烷基聚氧乙烯醚（Brij-35）对苯丙氨酸、精氨酸的NBD-Cl衍生物有荧光增敏效果。在含有5mmol/LBrij-35和20%乙腈添加剂的15mmol/L硼砂缓冲液（pH9.2）中,精氨酸、苯丙氨酸、丙氨酸、甘氨酸、谷氨酸及天冬氨酸等6种氨基酸的NBD-Cl衍生物可以在70s内实现分离,其中较难分离的丙氨酸、甘氨酸的NBD-Cl衍生物分离度为0.91;6种氨基酸衍生物的检出限为0.15～1.6μmol/L。

英文摘要：

The effect of various surfactants and solvents on the fluorescence properties of the 4-chloro-7-nitrobenzofurazan（NBD-Cl） derived amino-acids was studied.The experimental conditions for the derivatization reaction between NBD-Cl and amino acids,and those for chip-based capillary electrophoresis separation-laser induced fluorescence detection（CCE-LIF） of the amino acid derivatives were optimized.It was found that above their critical micelle concentrations,cationic surfactant CTMAB sensitized the fluorescence intensity of NBD-Cl derived arginine,glutamic acid,and aspartic acid,while non-ionic surfactant Brij-35 did the fluorescence intensity of the NBD-Cl derived phenylalanine and arginine.It was observed that six NBD-Cl derived amino acids of arginine,phenylalanine,alanine,glycine,glutamic acid and aspartic acid were CCE separated within 70 s in a 15-mmol/L pH 9.2 borate buffer containing 5 mmol/L Brij-35 and 20% acetonitrile additives,the resolution of the partially overlapped alanine and glycine derivatives being 0.91,and the detection limits of the six derived amino acids were 0.27-1.6 μmol/L.