中文摘要：

为明确福建地区是否存在鹅出血性多瘤病毒（goose hemorrhagic polyomavirus,GHPV）感染,本研究对2016年福建省临床送检测19份鸭组织样品进行GHPV检测,结果从1例樱桃谷鸭中检测到GHPV感染阳性（记为GHPV-FJ201601株）。随后根据GenBank中GHPV参考株序列特征,设计针对GHPV的VP3基因特异性引物,利用PCR技术扩增获得GHPV的VP3基因片段。结果显示,GHPV-FJ201601株的VP3基因全长为654bp,编码217个氨基酸。对编码的VP3蛋白分析发现其理论等电点为9.37,带负电荷氨基酸为23个（Asp＋Glu）,带正电荷氨基酸为28个（Arg＋Lys）;不稳定指数为38.43,是一个稳定蛋白;脂肪系数64.33,总平均疏水性指数为-0.744;该蛋白无典型的信号肽切割位点;其亚细胞定位类型为核定位;对其进行磷酸化分析发现,该蛋白存在14个氨基酸磷酸化位点。核苷酸同源性分析显示,不同来源GHPV代表株VP3基因相互之间核苷酸同源性均较高,均不低于99.7%。VP3基因遗传进化结果显示,GHPV-FJ201601株和鹅源GHPV分离株（匈牙利14234株与法国Toulouse Goose 2000株）处于同一遗传进化分支,与鸭源分离株（法国Toulouse Muscovy Duck 2008株、法国Toulouse Mule Duck 2008和中国106株）却处于不同的遗传进化分支,但所有GHPV分离株VP3基因遗传进化均较近。本研究首次证实福建地区樱桃谷鸭群中存在GHPV感染,为丰富不同地区与宿主的GHPV分子流行病学数据提供参考。

英文摘要：

In order to investigate goose hemorrhagic polyomavirus （GHPV） infection status in Fujian, China, 19 samples which were collected from different duck species were used to detecte GHPV, only one sample from Cheery Valley duck was tested positive （designated GHPV-FJ201601 strain）. VP3 gene specific primers were designed by comparing the characterization of GHPV genomic sequences based on the genomic sequences download from GenBank. The length of the cloned VP3 gene was 654 bp, coding 217 amino acids. The theoretical pI of VP3 protein was 9.37, with 23 total number of negatively charged residues （Asp ＋ Glu） and 28 total number of positively charged residues （Arg ＋ Lys）, respectively. The instability index of VP3 protein was 38.43, and it was a stable protein; The fat coefficient was 64.33; The total average hydrophobic index was -0.744. Also, the VP3 protein did not have signal peptides which was located in nucleus and having 14 phosphorylation sites. Nucleotides homology analysis of VP3 gene of GHPV isolates were higher with each other, which were no less than 99.7%. Phylogenetic analysis based on the VP3 gene showed that GHPV-FJ201601 strain shared the same genetic evolution cluster with goose-origin GHPV （14234 strain from Hungary and Toulouse Goose 2000 strain from France） rather than the duck-origin GHPV （Toulouse Muscovy Duck 2008 strain and Toulouse Mule Duck 2008 from France and 106 strain from China）, all VP3 gene shared closer genetic distance with each other. In conclusion, this study was first evidence of Cheery Valley duck could infect GHPV in Fujian, which laid a good foundation for the enrichment the GHPV epidemiology data from different area and host.