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中文摘要：
目的观察缺血预处理对大鼠脑缺血再灌注后x盒结合蛋白1（XBP-1）mRNA及其蛋白表达的影响。方法SD大鼠60只，随机分为假手术组（SO）、脑缺血再灌注组（MCAO）、脑缺血预处理组（BIP）3组，每组按照再缺血后12h、1d、2d、3d4个时间点分为4个亚组。采用二次线栓法制备大鼠局灶性脑缺血预处理模型，用实时荧光定量PCR和Westernblot法观察再缺血后各个时间点XBP-1mRNA及其蛋白的表达变化。结果MCAO组XBP-1mRNA及其蛋白表达均于缺血再灌注后12h开始明显上升，24h达高峰（P〈0．01），随再灌注时间延长其表达逐渐下降，但仍保持较高表达水平（P〈0．01）；BIP组较MCAO组XBP-1mRNA及其蛋白表达明显升高（P〈0．05，P〈0．01）。结论脑缺血预处理可能通过诱导XBP-1表达发挥其神经保护作用。
英文摘要：
Objective To investigate the effect of focal ischemic preconditioning（IPC） on the expression of X-box binding protein 1 （XBPol） mRNA and protein after focal cerebral ischemia/reperfusion（I/R） in rats. Methods 60 male SD rats were randomly divided into three groups：sham-operation group, middle cerebral artery occlusion （MCAO） group and brain ischemia preconditioning（BIP） group. Each group was further divided into 4 subgroups according to 12h, I d,2d and 3d after I/R. The BIP models were made in order to measure the expression of XBP-1 mRNA and protein by Real time PCR and Western blot. Results The expression both of both XBP-1 mRNA and its protein all increased and reached the peak at 24h, then decreased continuously（ P 〈 0.05 ）. BIP could increase their expression （ P 〈 0.05 ） P 〈 0.01 ）. Conclu- sion BIP could protect neurons through upregulating the expression of XBP-1 after endopiasmic reticulum stress in rats.
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