中文摘要：

研究了苯并[α]芘（B[α]P对栉孔扇贝血细胞存活率、活性氧（ROS）含量、溶酶体膜和DNA损伤以及凋亡指数的影响。结果表明：B[α]P对栉孔扇贝血细胞存活率无明显影响,存活率在96.72%～97.71%,而B[α]P对血细胞ROS含量、溶酶体膜稳定性和DNA损伤以及凋亡指数影响显著（p〈0.05）,且对照组无明显变化。各处理组血细胞ROS含量在实验时间内均表现出明显的上升趋势,且与B[α]P的作用时间呈正相关,同时在12、24 h时,各B[α]P处理组血细胞ROS含量大小为：1 mg/L〉4 mg/L〉16 mg/L〉0.25 mg/L。除0.25 mg/L处理组血细胞溶酶体膜稳定性、拖尾率和任意值在12 h和6 h内以及1 mg/L处理组血细胞溶酶体膜稳定性、拖尾率在6 h内与对照组无明显差异外,其余各处理组血细胞溶酶体膜稳定性以及拖尾率、任意值、凋亡指数在实验时间内分别呈现下降或上升趋势,同时各处理组血细胞溶酶体膜稳定性和DNA损伤程度、凋亡指数分别与B[α]P浓度和作用时间呈负、正相关性。因此,作者认为B[α]P对栉孔扇贝血细胞具有明显的免疫毒性损伤效应,且与B[α]P胁迫程度直接相关,同时B[α]P在血细胞内代谢产生的ROS及其代谢产物是造成血细胞损伤、凋亡的重要原因之一。

英文摘要：

The effects of benzo[α]pyrene（B[α]P）on livability,reactive oxygen species（ROS）content,lysosomal membrane and DNA damage as well as apoptosis index of haemocyte in scallop Chlamys farreri were studied.The results indicated that B／P had no effect on haemocyte livability,which was as high as 97.42%～98.82%.However B[α]P had significant effects on ROS content,lysosomal membrane stability,DNA damage and apoptosis index,while there was no notably change in control groups.The ROS content increased significantly in each B[α]P treated groups,and showed positive correlaction with the exposure time,and the ROS content was ＂1 mg/L〉4 mg/L〉16 mg/L〉0.25 mg/L＂ at 12 h and 24 h.Except of the lysosomal membrane stability,comet ratio and arbitrary value in 0.25 mg/L treatments within 12 h and 6 h,as well as lysosomal membrane stability,comet ratio in 1 mg/L treatments within 6 h had no significant change compared to control groups,the other treatment groups including lysosomal membrane stability as well as comet ratio,arbitrary value and apoptosis index decreased or increased during the experiment time,and which had negative or positive relationship with the exposure concentration and time of B[α]P.Therefore,the authors considered that the B[α]P had significant damage effects on haemocyte of scallop C.farreri,which had direct relationship with exposure extent of B[α]P.Meanwhile,the authors considered that the generation of ROS and the intermediate metabolite during the metabolism of B[α]P in haemocyte was one of the important reasons to cause the damage and apoptosis of haemocyte.