中文摘要：

以海洋微藻强壮前沟藻(Amphidinium carterae Hulburt)为研究对象,研究氮磷比对强壮前沟藻不同生长时期富集2,2’,4,4’–四溴联苯醚(BDE-47)的影响,测定其比生长率、脂类和碳水化合物等指标,分析了不同生长时期强壮前沟藻对BDE-47的富集与其生长和生化成分的关系。研究发现,在强壮前沟藻的适应期,氮磷比16时的强壮前沟藻细胞富集量为1.3 ng/(106 cells),显著低于其他氮磷比;在对数期和稳定期,氮磷比64、128时其富集量显著高于其他氮磷比,对数期氮磷比64和128的富集量可达2.5—2.6 ng/(106 cells),约是其他氮磷比的1.5倍;稳定期时约是其他氮磷比的2.4倍,且氮磷比128时高达3.6 ng/(106 cells)。不同氮磷比下强壮前沟藻对BDE-47的富集量与脂类含量之间呈一定的正相关关系,线性拟合回归系数近0.6。结果表明,营养盐结构导致了强壮前沟藻的生长和脂类等生化成分变化,并与BDE-47富集量呈正相关关系,从而可对强壮前沟藻富集BDE-47产生影响。

英文摘要：

In this study we explored the bioaccumulation of 2,2’,4,4’-Tetrabromodiphenyl ether (BDE-47) by Amphi-dinium carterae Hulburt under different N/P ratios (1, 4, 16, 64, 128) in different growth phases. Specifically we exa-mined the algal specific growth rate and the biochemical composition including lipid and carbohydrate, as well as the relationship between the amount of bioaccumulated BDE-47 and the biochemical composition. The results showed that the amount of bioaccumulated BDE-47 was 1.3 ng/(106 cells) when the N/P ratio was 16 in the lag phase which was significantly lower than those under other N/P ratios. In the log and stationary phase, the bioaccumulated BDE-47 under N/P ratios of 64 and 128 was significantly richer than those under other ratios. Under the two N/P ratios mentioned above, the amounts of bioaccumulated BDE-47 in the log [2.5-2.6 ng/(106 cells)] and stationary phases were 1.5 and 2.4 times higher than those under other N/P ratios respectively. It reached to 3.6 ng/(106 cells) under N/P ratio of 128 in stationary phases. There was a positive correlation between the amount of bioaccumulated BDE-47 and cellular lipid, with the R2 value of nearly 0.6. Our results showed that the lipid content was positively correlated with the bioaccumu-lation of BDE-47;therefore the N/P ratio could affect the amount of bioaccumulated BDE-47 by altering the lipid con-tent.