中文摘要：

基于黄瓜基因组重测序结果,搜索InDel位点,按照每隔1～3M个碱基对的距离选择并设计遍布全基因组的代表性InDel引物134对,以16份黄瓜典型种质检测其有效性。结果显示,134对引物均获得扩增产物,其中具有多态性的引物116对,占引物总数的86.6%。116对引物充分揭示出16份种质的多样性和特异性。本研究所开发的InDel分子标记将为黄瓜种质资源和分子遗传育种研究提供有力的遗传工具。

英文摘要：

In this study, 134 pairs of primers were designed by screening the InDel loci at a distance of every 1-3M base pairs on all chromosomes of cucumber based on genome re-sequencing. The effectiveness of the InDel markers were tested on 16 typical cucumber germplasms. The results showed that distinct PCR amplification prod- ucts were obtained from the InDel markers. Among these markers, 116 lnDel were polymorphic, accounting for 86. 6% of the total and revealing the genetic diversity and specificity of the tested cucumber germplasms. The InDel markers developed in the study would offer powerful tools for cucumber germplasm identification and marker-assis- ted seleetionin cucumber breeding.