中文摘要：

目的：探讨血管内皮细胞生长因子（VEGF）基因多态与血浆VEGF浓度以及脑卒中患病风险的关系。方法：采用PCR和限制性酶切片段长度多态性（RFLP）的方法，对1849例脑卒中患者和1798例对照者检测了VEGF基因启动子多态-460T／C、-116G／A和＋405G／C的频率分布。Logistic回归模型校正传统危险因素后分析多态与脑卒中的独立相关性。ELISA法检测血浆VGEF浓度。结果：脑卒中组血浆VEGF水平显著高于对照组[分别为（291±61．9）ng／L、（225±56．3）ng／L;P〈0．01]；VEGF基因多态-460T／C，-116G／A和＋405G／c与血浆VEGF浓度不相关（相关系数分别是：r=-0．119，P〉0．05；r=0．173，P〉0．05；r=0．144，P〉0．05）。未发现VEGF基因多态与脑卒中的患病风险具有相关性。结论：脑卒中组血浆VEGF水平升高支持VEGF在脑卒中发病过程中的作用，但VEGF基因5＇-调控区多态与脑卒中的易感性不相关。

英文摘要：

Objective：To assess the gene polymorphisms and concentration of vascular endothelial growth factor （VEGF） and the risk for stoke. Method：Total 1 849 patients with stroke （43.9 % cerebral atherothrombosis, 28. 7 % lacunar infarction, and 27.4 % intracerebral hemorrhage） and 1 798 controls were recruited from 7 centers. Three polymorphisms of -460T/C, -116G/A, and ＋405G/C in the VEGF gene were genotyped by PCR-RFLP assays. Multivariate logistic regression model was used to exclude the influence of conventional vascular risk factors on the risk of stroke. VEGF plasma level was measured by ELISA. Result：Plasma VEGF levels were significantly higher in cases than in controls [-291±61.9] versus [-225±56.3]ng/L, P〈0.01）. However, no significant correlation was found between the tested VEGF polymorphisms and VEGF levels （- 460T/C： r=-0.119,P=0.37;-116G/A; r=0.173,P=0.19;＋405G/C： r=0.144,P=0.27; respectively）. All VEGF markers were not associated with stroke after adjustment for established vascular risk factors. Concluion：These data suggested that elevated levels of plasma VEGF might play a role in the development of stroke, while polymorphisms in the 5＇-regulatory region of VEGF gene did not contribute to stroke.