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Molecular cloning and characterization of a threonine/serine protein kinase lvakt from Litopenaeus vannamei

ISSN号：0254-4059
期刊名称：《中国海洋湖沼学报：英文版》
时间：0
分类：Q785[生物学—分子生物学] S968.22[农业科学—水产养殖;农业科学—水产科学]
作者机构：[1]Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen361005, China
相关基金：Supported by the Scientific Research Foundation of Third Institute of Oceanography, SOA （No. 2011018）, the Natural Science Foundation of Fujian Province of China （No. 2011 J05079）, the National Natural Science Foundation of China （No. 31001125）, the Major State Basic Research Development Program of China （973 Program）（No. 2012CB 114403）, the National High Technology Research and Development Program of China （863 Program）（No. 2012AA092205）, and the China Agriculture Research System-47

关键词：凡纳滨对虾, 分子克隆, 蛋白激酶, 鉴定, 丝氨酸, 苏氨酸, 磷脂酰肌醇3-激酶, 实时定量PCR, AKT, Litopenaeus vannamei, white spot syndrome virus （WSSV）, inhibitor

中文摘要：

phosphatidylinositol 3-kinase (PI3K )-AKT 小径涉及各种各样的细胞的功能，包括骑车的 anti-apoptosis，蛋白质合成，葡萄糖新陈代谢和房间。然而，在甲壳纲的动物的 PI3K-AKT 小径的角色仍然保持不清楚。在现在的学习，我们从 Litopenaeus vannamei 克隆并且描绘 AKT 基因 lvakt。511 残余 LVAKT 高度被保存；包含了一个 PH 领域，一个催化领域和一个恐水病的领域；并且高度在 L 的心和鳃被表示。vannamei。我们发现用实时量的 PCR (Q-PCR ) 分析，那 lvakt 在早白色期间是起来调整的点症候群病毒(WSSV ) 感染。而且， PI3K 特定的禁止者， LY294002，减少的病毒的基因抄写，暗示 PI3K-AKT 小径可能被 WSSV 劫持。我们的结果因此建议 LVAKT 可以对 WSSV 在虾免疫者反应起一个重要作用。

英文摘要：

The phosphatidylinositol 3-kinase （PI3K）-AKT pathway is involved in various cellular functions, including anti-apoptosis, protein synthesis, glucose metabolism and cell cycling. However, the role of the PI3K-AKT pathway in crustaceans remains unclear. In the present study, we cloned and characterized the AKT gene lvakt from Litopenaeus vannamei. The 511-residue LVAKT was highly conserved; contained a PH domain, a catalytic domain and a hydrophobic domain; and was highly expressed in the heart and gills of L. vannamei. We found, using Real-Time Quantitative PCR （Q-PCR） analysis, that lvakt was up- regulated during early white spot syndrome virus （WSSV） infection. Moreover, the PI3K-specific inhibitor, LY294002, reduced viral gene transcription, implying that the PI3K-AKT pathway might be hijacked by WSSV. Our results therefore suggest that LVAKT may play an important role in the shrimp immune response against WSSV.

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