中文摘要：

目的建立基于PCR-LDR平台的近交系小鼠SNP快速分型方法,用于检测实验小鼠的遗传质量与品系纯度。方法利用可移植性极高的PCR-LDR技术,以常见近交系小鼠为研究对象,选取了21条染色体上的45个SNP位点,分别设计引物和探针,经过筛选和验证,建立了多重PCR-LDR（polymerase chain reaction and ligase detection reaction,PCR-LDR）分型方案。结果四组多重PCR-LDR可实现45个SNP位点的基因分型,其中43个、44个与45个SNP在样本中的检出率分别为100%、90.9%与36.4%。所有样本经分型确定为纯合体,并得到了常见近交系小鼠SNP位点信息。结论实现了常见近交系小鼠快速、高通量的基因分型,可用于遗传质量检测和品系鉴定。

英文摘要：

Objrctive Purity of laboratory animals plays a critical role affecting the experimental results. The aim of this study was to validate a high-throughput genotyping platform, valuable in inbred mouse genetic DNA monitoring and strain identification. Methods Multiple polymerase chain reaction and ligase detection reaction （PCR-LDR） genotyping panels were constructed. Forty-five single-nueleotide polymorphism （SNP） on 21 chromosomes were selected as targets, as well as specific primers and probes to these SNP were designed, respectively. A multiple PCR-LDR genotyping protocol was established. Results Forty-five SNP were successfully genotyped in four panels. The positive detection rate of 43, 44 and 45 SNPs were 100% , 90.9% and 36.4% , respectively. All samples collected were homozygous and their genotypes were identified by PCR-LDR. Conclusion The results of this study provide a rapid and high-throughput genotyping approach, which is sufficient for genetic contamination monitoring and strain identification.