中文摘要：

目的：研究细胞因子信号转导负调控蛋白3A（suppressor of cytokine signaling 3A,SOCS3A）在多疣壁虎脊髓中的细胞定位及过表达SOCS3A对神经元和星形胶质细胞生物学行为的影响。方法：采用组织免疫荧光方法检测壁虎断尾脊髓中SOCS3A的细胞定位;构建SOCS3A腺病毒,分别在SH-SY5Y神经元细胞株和GSN1壁虎星形胶质细胞株中转染SOCS3A过表达腺病毒,观察对神经元轴突生长及JAK-STAT3的信号通路的影响,对星形胶质细胞增殖及迁移的影响。结果：免疫荧光染色结果表明,SOCS3A与多疣壁虎脊髓中的神经元和星形胶质细胞有共定位。过表达SOCS3A对神经元轴突生长无明显抑制作用,对JAK-STAT3的信号通路无显著影响;过表达SOCS3A可抑制星型胶质细胞的迁移,但对细胞增殖没有明显影响。结论：在多疣壁虎断尾脊髓再生过程中,SOCS3A与神经元和星形胶质细胞的功能相关,不参与JAK-STAT3信号通路发挥调节作用。

英文摘要：

Objective： To investigate the tissue distribution of suppressor of cytokine signaling 3A（SOCS3A） in the spinal cord, and to examine the effects of gecko SOCS3A on the cellular events of neurons and astrocytes. Methods： Immunohistochemistry was employed to detect the tissue distribution of SOCS3A in the spinal cord; neuronal cell line SH-SY5Y and gecko astrocyte cell line GSN1 were transfected with adenovirus of SOCS3A. Results： Immunostaining demonstrated that SOCS3A colocalized with neurons and astrocytes in the gecko spinal cord. Overexpression of SOCS3A did not significantly alter neuronal axon growth, but inhibited the migration of astrocytes without effects on cellular proliferation. SOCS3A overexpression displayed no obvious effect on JAK-STAT3 signaling pathway. Conclusion： SOCS3A is related to the function of neurons and astrocytes, and is not involved in the regulation of JAK-STAT3 signaling pathway, during the gecko spinal cord regeneration following tail amputation.