中文摘要：

polyphenoloxidase (PPO ) 是考虑为蘑菇 browning 负责的关键酶。由使用克隆的相同和 cDNA 结束(种族) 的快速的扩大，二新 PPO 基因和相应 cDNA 从 Agaricus bisporus 的水果身体被识别(AbPPO3 和 AbPPO4， GenBank 就职 nos. GU936494 和 GU936493，分别地) 。genomic DNA AbPPO3 定序，分别地， AbPPO4 是在长度的 2 080 和 2 189 bp 编码约 66 和 68 kDa 的通常认为的多肽。二个铜绑定领域的推出的氨基酸序列表演特征特征包括真菌的多酚 oxidases 在类型 III 铜蛋白质保存了。顺序比较显示 AbPPO3 和 AbPPO4 介绍 55.3% 类似给对方(48% 身份) 。我们也多于 1.5-kb 获得了 AbPPO3 和 AbPPO4 的开始 codon 在上游、认出的长序列他们作为他们的各自的通常认为的倡导者。二个 PPO 倡导者区域的分析证明他们包含为厌氧的正式就职，光，创伤，应力，和植物生长素反应可能负责的丰富的行动 cis 元素。半量的 RT-PCR 结果显示 AbPPO3 和 AbPPO4 高度在成熟水果身体被表示并且在 2-d 存储以后起来调整采蘑菇。这些结果建议 AbPPO3 和 AbPPO4 可以在 A 起作用。在在倡导者的开发和 postharvest 存储和元素期间的 bisporus， browning 和色素沉着可以为 AbPPO3 和 AbPPO4 的可诱导的表示充当规章的元素。成功的克隆和 AbPPO3 和 AbPPO4 保证的表达式分析 A 的结构和函数上的进一步的调查。为基因操作指向可能的目标的 bisporus PPO。

英文摘要：

The polyphenoloxidase（PPO） is the key enzyme considered to be responsible for mushroom browning.By using homology cloning and rapid amplification of cDNA ends（RACE）,two new PPO genes and the corresponding cDNA were identified from the fruit bodies of Agaricus bisporus（AbPPO3 and AbPPO4,GenBank accession nos.GU936494 and GU936493,respectively）.The genomic DNA sequences of AbPPO3 and AbPPO4 are 2 080 and 2 189 bp in length,respectively,encoding putative polypeptides of approximately 66 and 68 kDa.The deduced amino acid sequences show characteristic features of two copper-binding domains conserved in the type III copper proteins including fungal polyphenol oxidases.Sequence comparisons indicate that AbPPO3 and AbPPO4 present 55.3% similarity to each other（48% identity）.We also obtained more than 1.5-kb long sequences upstream of the start codon of the AbPPO3 and AbPPO4 and recognized them as their respective putative promoters.Analyses of the two PPO promoter regions show that they contain abundant cis-acting elements which are probably responsible for anaerobic induction,light,wound,stress,and auxin response.Semi-quantitative RT-PCR results indicate that AbPPO3 and AbPPO4 were highly expressed in the mature fruit bodies and up-regulated after 2-d storage of mushroom.These results suggest that AbPPO3 and AbPPO4 may play roles in A.bisporus browning and pigmentation during development and postharvest storage and the elements in promoters may act as regulatory elements for the inducible expression of AbPPO3 and AbPPO4.The successful cloning and expression analysis of AbPPO3 and AbPPO4 warrant a further investigation on the structure and function of A.bisporus PPO which points to the possible targets for genetic manipulation.