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中文摘要：
目的研究纤维蛋白胶与人牙囊细胞（DFCs）二维（2D）及三维（3D）结合模式对细胞存活、增殖及凋亡的影响，评估纤维蛋白胶作为细胞移植载体的可行性及有效性。方法采用细胞膜绿色荧光探针（DIO）对存活DFCs细胞膜进行标记，采用ImageJ2x软件细胞计数；采用碘化丙啶（PI）对凋亡细胞进行染色。结果纤维蛋白胶在3D-2和2D．1复合模式下的降解速度快于3D-1模式；纤维蛋白原浓度为15mg·mL。辅以凝血酶浓度2U·mL。条件下，DFCs能够存活并生长良好，尤其在3D-l结合模式下更有利于细胞增殖及伸展。结论纤维蛋白胶可作为一种有效的细胞移植载体，纤维蛋白胶不同的结合方式对细胞的增殖活性有一定的影响，3D-1复合模式更有利于细胞的存活增殖。
英文摘要：
Objective This study explores the effects of different fibrin glue combination modes on the survival, prolife- ration, and apoptosis of dental follicle cells （DFCs）, as well as to evaluate the feasibility and effectiveness of fibrin glue as trans- plantation material. Methods The membranes of surviving DFCs were marked using 3,3＇-dioctadecyloxa carbocyanine per- chlorate （DIO）, and the cell number was counted by using ImageJ2x software. The apoptotic cells were marked with prodium iodide （PI）. Results Compared with that of the 3D-2 and 2D-1 groups, the degradation speed of the 3D-1 group was the slowest. DFCs could survive and grow well in fibrinogen with a concentration of 15 mg＇mL-a supplemented with thrombin with a concentration of 2 U＇mL-1. In particular, the 3D-1 combination mode was significantly conducive to cell proliferation and stretching. Conclusion Fibrin glue can be used as an effective cell transplantation material. The different combination modes have certain effects on cell proliferation. The 3D-1 combination mode is more conducive to the survival and prolifera-tion of DFCs than other modes.
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