以"盛丰"品种白萝卜为试材,采用不同浓度(0.1%、0.3%、0.5%)壳聚糖溶液对萝卜种子进行处理,研究了种子萌发后不同生长天数(3、5、7d)幼苗的生理生化指标(叶绿素、类胡萝卜素、总酚、芥子油苷、DPPH抑制率、PAL活性、CAT活性、POD活性)的变化情况,以探求壳聚糖处理萝卜种子后对其幼苗生理生化特性的影响。结果表明:与对照组(未进行壳聚糖包衣处理)相比,采用壳聚糖处理对萝卜幼苗的生理生化指标均有显著影响。对于生长3d和5d的幼苗,3种浓度的壳聚糖处理均能显著提高其叶绿素、类胡萝卜素、总酚、芥子油苷含量;而对于生长7d的幼苗,只有0.3%和0.5%壳聚糖处理能显著提高其叶绿素、类胡萝卜素、总酚、芥子油苷含量;3种浓度壳聚糖处理均能显著提高生长3、5、7d的幼苗CAT活性及生长5d的幼苗PAL活性;0.1%壳聚糖处理能提高生长3d的幼苗POD活性,0.5%壳聚糖处理能提高生长5、7d的幼苗POD活性。表明采用壳聚糖处理萝卜种子能提高其幼苗营养物质含量、改善品质,对农业生产具有一定的指导意义。
Adopting"Shengfeng"white radish as experimental material,seeds were coated with different concentrations(0.1%,0.3%,0.5%)of chitosan.The variation of physiological indexes of 3-,5-and 7-day-old sprouts(chlorophyll,carotenoid,total phenolic,total glucosinolate,DPPH inhibition ratio,PAL activity,CAT activity and POD activity)were investigated,to investigate the effect of chitosan treatment on sprouts physiological property of radish.The results showed that the effect of chitosan treatment on sprouts had a significant difference in comparison to the control sprouts.For the3-and 5-day-old sprouts,the contents of chlorophyll,carotenoid,total phenolic and total glucosinolate were significantly enhanced under 0.1%,0.3% and 0.5% of chitosan treatment.For the 7-day-old sprouts,the contents of chlorophyll,carotenoid,total phenolic and total glucosinolate were significantly increased under 0.3% and 0.5% of chitosan treatment.Besides,the CAT activity of 3-,5-and 7-day-old sprouts and the PAL activity of 5-day-old sprouts were significantly increased under 0.1%,0.3%and 0.5%of chitosan treatment.The POD activity of 3-day-old as well as 5-,7-day-old sprouts was significantly enhanced under 0.1%and 0.5%of chitosan treatment,respectively.It showed the nutritional value of radish sprouts grown from the seeds coated with chitosan could be improved,which could provide guidance for agricultural production.