中文摘要：

目的检测良性前列腺增生（BPH）与前列腺癌（PCa）组织标本HER-2及C—mycmRNA相对值，探讨此值对PCa诊断的特异性意义。方法通过实时荧光定量PCR检测63例PCa、37例BPH及3例正常前列腺组织HER-2及C-mycmRNA的表达，比较其在PCa与BPH中组织定量的差异。结果相对于BPH，PCaHER-2及C-mycmRNA表达相对值分别为4．25±0．03，7．24±0．06，PCaHER-2及C-myc表达相对值较BPH高，差异有统计学意义（均为P〈0．05）。结论实时荧光PCR定量检测HER-2及c-mycmRNA为PCa的诊断提供了重要的辅助指标。

英文摘要：

Objective To study the HER- 2/C- myc mRNA expression in benign prostatic hyperplasia （BPH） and prostate cancer （PCa） tissue, and to explore the value of HER-2/C-myc mRNA expression in PCa diagnosis. Methods A sensitive, real-time fluorescence quantitative （FQ） PCR assay was used to detect the differential expression of HER-2 and C- myc mRNA in prostate samples from 63 patients with PCa, 37 with BPH and 3 healthy controls. Results The expression levels of HER-2 （4.25＋ 0.03） and C-myc （7.24＋0.06） in PCa tissue were significantly higher than those in BPH （both P〈0.05）. Conclusion The detection of HER-2 and C-myc mRNA expression by FQ-PCR may provide reliable and helpful information for the diagnosis of PCa.