中文摘要：

快速碱化因子是近年来新发现的一种植物多肽类信号分子,广泛存在于高等植物中。通过已得到的普通白菜的快速碱化因子基因BcMF14（GenBank序列登录号EF523516）的核苷酸序列,在其编码框两侧设计引物,从菜心中克隆出该类信号分子基因,命名为BcRALF（登陆号：GU086228）。序列同源比对表明该基因与花椰菜、拟南芥等的快速碱化因子基因有很高的相似性,证明BcRALF属于快速碱化因子家族。蛋白质特征预测以及蛋白序列结构分析发现BcRALF蛋白包含有多个生物活性位点,符合其作为多肽类信号分子类蛋白的特征。

英文摘要：

The rapid alkalinization factor （RALF） gene is a new found plant polypeptide signal molecule,wide spreading in higher plants.In this study BcRALF was cloned from Brassica campestris ssp.chinensis Makino var.parachinensis based on BcMF14 （GenBank accession number EF523516） from B.campestris ssp.chinensis var.communis cv.Aijiaohuang.The sequence of this gene was 273 bp （GU086228） and was identified as a rapid alkalization factor gene according to its high identity with B.oleracea var.botrytis BoRALF1 and Arabidopsis thaliana RALFL9.Protein characteristics and sequence structure were predicted,and moreover,many bioactive sites were found.The results showed that the characteristics of the BcRALF protein consistent with its category as a peptide signal molecule.