中文摘要：

近十多年来，以基因芯片为代表的阵列芯片技术以其高通量的优点在生物、检疫、制药等领域中得到广泛应用．由于原位合成技术专利为Affymatrix等少数公司拥有且少量制作时成本过高，目前普遍采用合成后点样技术制备阵列芯片，即将含有探针分子的小液滴分散到固体基片表面，蒸发后形成微点阵列．由于“咖啡环效应”，

英文摘要：

Microarray chip including genechip has been of great application in fields of biology, quarantine, pharmacy and so on. Most of microarray chips were prepared by spotting aqueous droplet on the surface of substrate. Due to ＂coffee-ring stain effect＂, there always was a denser perimeter where sample was concentrated during the process of droplet drying after spotting. So the ununiformity of spot caused by ＂coffee-ring stain effect＂ was one troublesome problem of the chip quality. Herein, superhydrophilie spot arrays on superhydrophobic AAO surface were fabricated to ease the ＂coffee-ring stain effect＂. Furthermore, a protein array is prepared on this patterned surface where immunoreactions are carried out. As a result, spot uniformity is greatly improved in a wide range of concentration and volume.