中文摘要：

为了探讨DNA条形码技术在花卉害虫防治时用于蚜虫物种的快速、准确鉴定的可行性,本研究利用条形码通用引物扩增了危害广州市常见菊花品种的2种蚜虫,桃蚜Myzus persicae（Sulzer）和棉蚜Aphis gossypii Glover的DNA条形码。通过分析获取的2种43个样本的线粒体细胞色素C氧化酶亚基I（mitochondrial cytochrome c oxidase subunit I,COI）基因658 bp序列后发现,碱基平均含量为39.8%T,14.3%C,35.3%A,10.6%G,其中A＋T含量为75.1%,C＋G含量为24.9%,存在明显的A、T碱基偏好性,符合昆虫线粒体基因碱基组成的基本特征;有保守位点551个,可变位点62个,简约位点56个,单个突变位点6个。序列饱和性检验分析结果显示该序列转换与颠换未达到饱和,因此可以进行基于Kimura双参数模型的种内种间遗传距离分析。所有样品的种内平均差异为0.08%（0.00%~1.20%±0.18%）,种间平均序列差异为9.69%（9.60%~10.30%±0.13%）。同时,邻接法（neighbor-joining,NJ）构建的系统发育树显示采自不同地区不同菊花品种上的桃蚜和棉蚜分别聚为一支。因此,本研究证明应用基于COI基因片段的DNA条形码进行花卉常见蚜虫类害虫的快速鉴定具有可行性。

英文摘要：

In order to identify the aphids rapidly and accurately in flower pest control. DNA barcoding based on COI （mitochondrial cytochrome e oxidase subunit I） genes were used to analyze aphids on Chrysanthemum plants in Guangzhou. The 658 bp COl sequences of 43 samples belonging to 2 species, Myzus persicae （Sulzer） and Aphis gossypii Glover, in Guangzhou were amplified using universal barcoding primers. Among all sites, the average proportions of base were 39.8% T, 14.3% C, 35.3% A, and 10. 6% G respectively. It showed an obvious preference to A ＋ T in the sequence, that A ＋ T base pair accounts （75.1%） was significantly higher than that of G ＋ C base pair （24.9%）. The percentage of A and T was conforming to the basic feature of base composition in insect mitoehondria. The results of sequence analysis revealed that there are 551 conserved sites, 62 mutation sites, 56 parsimony - informative sites, and 6 singletons in this gene. The results of transition and transversionshowed that the sequence did not reach the saturation and the evolution analysis can be achieved accurately. Model Kimura 2 - parameter was used to analyze the genetic distance, and the results showed that the average intraspecies sequence divergences is 9.69% （9.60% - 10.30% ±0. 13% ）, while the average interspecies divergences is 0. 08% （0. 00% -1.20% ±0. 18% ）, confirming that the genetic distance can be used to discriminate different aphid species. Neighbor -joining phylogenetic tree was built based on the COI gene sequences, and the results indicated that the same species was gathered in the same branch. It suggested that DNA barcoding based on mitochondrial COI gene sequences is applicable in the classification and identification of common Chrysanthemum aphid species in Guangzhou City.