中文摘要：

目的明确Plunc基因AF172993序列是complete CDS还是transcript variant。方法利用RT-PCR方法扩增Plunc基因CDS区编码序列,将该序列克隆入pEGFP-N1真核表达载体中。正反双向测序分析,所得序列除了与AF172993序列两两比对,还与nr和人类基因组数据库进行比对分析。结果新克隆序列在CDS区658位C取代了AF172993序列A,遗传密码子由AAG改变成CAG,相应氨基酸编码也从Gln变为Lys。与人类基因组比对,Plunc基因CDS区658位C碱基能与人类20号染色体基因组2094188位C碱基完全匹配。结论AF172993序列658位A为突变位点,改变了氨基酸编码,实际为Plunc基因的变异体。GenBank数据库注释该序列错误。

英文摘要：

Objective To determine AF172993 sequence is either the complete CDS or a transcript variant. Methods RT-PCR was used to amplify the CDS sequence of Plunc, which was subsequently cloned into the pEGFP-N1 eukaryotic expression vector. After bi-directional sequence analysis, the sequence obtained was blasted against the AF172993 sequence, nr database and human genome database. Results In CDS of the new cloned sequence, the 658 base A in the AF172993 sequence was replaced by C, and the corresponding genetic code was also converted fi＇om AAG to CAG, leading to the alteration of the amino acid Gin to Lys. In addition, the base C at the 658 position of the CDS showed perfect match with the base C at 2094188 position in human chromosome 20. Conclusion The base A at the 658 position of AF172993 sequence of Plunc is a mutation site, which alters the coding of the amino acid. AF172993 sequence is actually a transcript variant of Plunc, and the annotation to AF172993 in GenBank database is not correct and need to be revised.