中文摘要：

以107速生杨（Populus×euramericanacv.‘74/76’）幼苗为材料,考察了接种丛枝菌根真菌（AMF）和外生菌根真菌（ECMF）幼苗在受到溃疡病菌侵害后生理生化指标和抗病相关蛋白的变化,初步探讨菌根真菌诱导植物抗病性的可能机制。结果表明,两种菌根真菌都能够显著降低杨树幼苗溃疡病的感病指数和发病率,显著提高杨树叶片叶绿素、树皮可溶性蛋白含量和根系活力强度;两种菌根真菌都可以显著降低杨树感病部位的丙二醛含量和提高游离脯氨酸含量,增强杨树超氧化物歧化酶（SOD）和苯丙氨酸解氨酶（PAL）的活性;丛枝菌根真菌能显著诱导提高杨树感病部位几丁质酶和-β1,3-葡聚糖酶活性,而外生菌根真菌则不能诱导或效应较低,且丛枝菌根真菌诱导杨树提高抗病性的相关指标均优于外生菌根真菌,而苗木感病指数和发病率均低于外生菌根真菌,其在幼苗期接种提高杨树抗溃疡病的效果较好。可见,在杨树受到溃疡病菌侵染时,菌根真菌可促进杨树对营养物质的吸收和生长,提高菌根苗体内保护酶活性和抗性相关化合物的含量,促使产生大量几丁质酶和-β1,3-葡聚糖酶来诱导杨树产生二次防卫反应,从而补偿病害所带来的损伤,增强抗病性。

英文摘要：

This study investigated the changes of biochemical index and the proteins associated with disease resistance after inoculated with arbuscular mycorrhizal fungi（AMF） of Glomus mosseae and ectomycorrhizal fungi（ECMF） of Boletus luridus on the growth of healthy poplar 107（Populus×euramericana cv.＇74/76＇） and the poplar attacked by canker disease.The possible mechanism of disease resistance induced by mycorrhizal fungi was primarily discussed.The results indicated that both AMF and ECMF significantly decreased the index and incidence of poplar canker disease and increased the content of chlorophyll,bark soluble protein content and root activity.In addition,both AMF and ECMF can decrease the content of malonaldehyde（MDA） and increase the free proline contents.The activity of superoxide dismutase（SOD） and phenylalnine ammonialyase（PAL） in diseased sites.AMF significantly induced the boost of activity of chitinase and β-1,3-glucanase in diseased sites,but ECMF just had a little effect.The index related with resisting canker disease among AMF was superior to ECMF,but both the incidence and disease index of canker disease of AMF were lower than ECMF,the effect of AMF was better when inoculating the poplar seedlings.It is clear that mycorrhizal fungi can improve the absorption of nutrient and growth of poplar when attacked by canker pathogen,the activity of protective enzyme and resistant compound were increased in the mycorrhizal poplar seedlings,producing large amount of chitinase and β-1,3-glucanase to induce secondary defending reaction of poplar.Consequently,mycorrhizal fungi can remedy the harm of disease and improve the disease resistance of poplar.