中文摘要：

目的：分析柑橘、油菜和紫云英蜜中的特征黄酮类组分。方法：采用UPLC—MS／MS同时分析油菜蜜、紫云英蜜和柑橘蜜及其蜜源花中的黄酮类组分。色谱柱采用Inertsil ODS-3柱（2．1mm×75mm，2μm），柱温40℃，以甲醇-0．02％甲酸为流动相梯度洗脱，流速为0．4mL·min-1；以儿茶酸、表儿茶酸、芦丁、芸香柚皮苷、桑黄素、杨梅素、山柰酚、生松素等16个黄酮类对照品作为对照，采用电喷雾离子源（ESI），多反应监测（MRM）进行分析。结果：不同蜜源蜂蜜中的特征黄酮类组分及其含量（从高到低排列）有所不同：柑橘蜜的特征组分有柚皮素、木犀草素、芹菜素和汉黄芩素；油菜花蜜的特征组分有柚皮素、槲皮素和汉黄芩素；紫云英蜜的特征组分有槲皮素、山柰酚、芹菜素和汉黄芩素。结论：该方法灵敏度高，以蜜源花作为“对照”寻找蜂蜜中的特征成分具有数据可靠、可溯源性，并对蜜源花中化学成分的分析具有指导意义。

英文摘要：

Objective： To analyze the characteristic flavonoids in citrus, canola flower and milk vetch honeys. Methods： The flavonoids in canola flower honey, milk vetch honey, citrus honey and their nectar flowers were simultaneously analyzed by UPLC-MS/MS. The samples were analyzed on an Inertsil ODS-3 column （ 2.1 mm×75mm,2μm ）, eluted with methanol-0.02% formic acid solution as mobile phase in gradient mode at a flow rate of 0.4 mL min-1, the column temperature was controlled at 40 ℃ and the injection volume was 2 μL; electrospray ionization （ ESI ） source was applied and operated in the negtive or positive multiple reaction monitoring （ MRM ） mode, 16 flavonoids were used as reference, such as catechin, epicatechin, rutin, narirutin, morin, myricetin,kaempferol, pinocembrin etc. Results： The characteristic components and their contents （ from highest to lowest ） were different in different nectar honey： characteristic components in citrus honey were naringenin, luteolin, and apigenin wogonin; canola flower honey had naringenin, quercetin and Wogonin; and milk vetch honey had quercetin, kaempferol, apigenin and wogonin. Conclusion： The method with flowers as a ＂control＂ to find honey＇ s characteristic component is sensitive, reliable, and traceable. The developed method was helpful to analyze the chemical components in flower nectar.