采用传统的子实体转接法和反复纯化法,从新疆阿克苏地区盐碱地土壤样品中分离1株粘细菌,经过对其菌落、子实体、营养细胞、粘孢子大小形态观察、生理生化特征试验及16SrDNA序列分析,鉴定菌株5x1为大孢珊瑚球菌(Corallococcus macrosporus),在对其进行耐盐性分析时发现,菌株5x1能耐受NaCl的浓度为2%。结果表明:菌株5x1为陆生耐盐粘细菌。
Base on conventional isolational method, a salt-tolerance myxobacteria was isolated from the saline-alkaline soils of Akesu in Xinjiang. According to the morphologies of swarms, fruiting bodies, vegetative cells, myxospores, physiological and biochemical characteristic and the sequence of 16S rDNA, the strain was identified to Corallococcus macrosporus. Analysis of salt- tolerance showed the strain 5xl could survive in NaCl concentration of 2%.