中文摘要：

为深入了解脂氧合酶基因在油茶果实成熟过程中的功能及其对油茶油脂品质的影响,本研究以油茶种子的转录组数据库中的脂氧合酶基因（lipoxygenase,LOX）的部分cDNA序列为基础,采用RT-PCR和RACE技术从油茶种子中克隆到此基因的全长cDNA,并其进行了序列分析。该基因全长cDNA为2 698 bp,包括5’非编码区161 bp、3’非编码区131 bp以及一个长2 406 bp的编码区和16 bp的polyA尾巴。该基因编码蛋白质的分子量约为91.65 kDa,等电点为5.255。同时该蛋白二级结构以α-螺旋为主,具有跨膜结构,主要分布在细胞内的叶绿体和细胞质中。经过比对分析,发现油茶LOX基因序列与其他物种LOX基因同源性较高,其编码的氨基酸序列与茶树、葡萄、辣椒、马铃薯的LOX氨基酸序列的同源性高达99%,与油橄榄、烟草、番茄、猕猴桃同源性达到了98%。

英文摘要：

In order to reveal the role of LOX gene in the development of Camellia oleifera seeds and the impact on seeds oil quality, a full-length eDNA of the LOX gene was cloned from C. oleifera seeds on the basis of transcriptome library with RT-PCR and RACE methods. The bioinformatics analysis suggested that the 2 698 bp eDNA was composed of a 2 406 bp ORF, a 161bp 5＇ UTR and a 131bp 3＇ UTR that contained a 16 bp polyA. The characters of Co-LOX protein showed the relative molecular mass was 91.65 kDa, the pI was 5.255, and a transmembrane domain was predicted in the second structure of the protein constructed mainly by a-helices. The distribution analysis results indicate that the protein appeared in chloroplast and cytoplasm. The phylogenetic investigation displays that Co-LOX shared homology with those of other species reaching up to 99%, such as C. sinensis, Vitis vinifera, Capsicum oleoresin, and Solannum tuberosum, and the relationship of Co-LOX and Olea europaea, Nicotiana tabacum, Lycopersicon esculentum and Actinidia chinensis reached up to 98%.