中文摘要：

目的：寻找内吗啡肽2（EM2）和μ阿片受体（MOR）在内脏感觉和运动调控过程中发挥作用的形态学证据。方法：（1）麦芽糖凝集素（WGA）示踪与免疫荧光组织化学染色技术相结合的多重标记方法；（2）用稀盐酸刺激胃，制作内脏痛动物模型；（3）免疫荧光组织化学多重染色方法。结果：（1）将WGA注入大鼠胃壁，在结状神经节（NG）内可见WGA标记神经元和WGA／EM2／MOR三标神经元；在孤束核（NTS）可见WGA跨节标记的纤维和终末以及WGA／EM2／MOR三标纤维和终末；在迷走神经运动背核（DMV）可见WGA逆标神经元和WGA／MOR双标神经元及其与EM2阳性纤维或终末形成的紧密接触。（2）将稀盐酸注入胃后，NG和NTS神经元内的FOS表达明显增多；在NG内可见EM2／FOS双标神经元；在NTS内可见EM2阳性纤维和终末与SP受体／FOS双标神经元形成紧密接触。结论：EM2和MOR可能影响内脏感觉和运动功能。

英文摘要：

Objective ： To look for morphological evidence for endomorphin 2 （EM2） and μ opioid receptor （MOR） exert modulatory effects on the visceral sensation and movement. Methods ： （ 1 ） Wheat-germ agglutinin （ WGA ） tracing combined with immunofluorescence histochemical staining; （2） Intragastric perfusion with low concentration of hydrochloric acid was used to make visceral pain model; （3） Immunofluorescence histochemical multi-staining. Results： （ 1 ） After injecting WGA into the stomach wall in the rat, WGA retrogradely labeled neurons and WGA/EM2/MOR triple-labeled neurons were observed in nodose ganglion （NG）. WGA transganglionically labeled fibers and terminals and WGA/EM2/ MOR triple-labeled fibers and terminals were observed in solitary tract nucleus （NTS）. WGA retrogradely labeled neu- rons and WGA/MOR double-labeled neurons and close contacts between WGA/MOR double-labeled neurons and EM2 immunopositive fibers and terminals were observed in dorsal motor nucleus of the vagus nerve （DMV）. （2） After intra- gastric perfusion with hydrochloric acid, FOS protein expression in neurons of the NG and NTS increased markedly ; EM2/ FOS double-labeled neurons were found in the NG; close contacts between substance P receptor （SPR）/FOS double labeled neurons and EM2 immunopositive fibers and terminals were observed in NTS. Conclusion： EM2 and MOR might be involved in modulation of the visceral sensation and movement.