中文摘要：

生长素极性运输基因PIN（PIN-FORMED）决定了植物中生长素浓度梯度分布,参与多种发育及次生代谢过程。本研究采用转录组热图聚类方法分析了4年生吉林人参14个不同部位PIN2、PIN3及PIN6的表达谱,结果显示PgPIN2与PgPIN6主要在人参地下根与根茎中表达,PgPIN3在人参的叶子、果实及根中均有很高的表达;采用序列同源比对、RT-PCR扩增测序及生物信息学分析方法,确认了人参3个PIN基因成员PgPIN2、PgPIN3与PgPIN6;采用实时荧光定量PCR技术分析人参不定根、组培苗不同部位的表达模式,发现PgPIN3与PgPIN6在人参不定根根尖表达高,PgPIN2在人参组培苗根部表达高,PgPIN3在人参组培苗的叶中表达高。人参PIN基因的组织特异性表达表明PgPIN2、PgPIN3与PgPIN6对根的生长发育及向性生长很可能具有相应的功能,PIN3对人参植株地上部分的生长具有较重要的意义,可为后期深入挖掘其对人参向性生长等功能奠定基础。

英文摘要：

Polar auxin transport gene PIN （PIN-FORMED） determines the concentration gradient of auxin and plays an important role in development and secondary metabolism of plants. This study was designed to analyze the bioinformatics and expression of the PIN genes in Panax ginseng to explore a novel way of breeding ginseng varieties. Heatmap and cluster analysis of PIN2, PIN3, PIN6 was performed in four-year-old Jilin ginseng. Sequence homology alignment, RT-PCR amplification, sequencing and bioinformatics analysis were used to identify three PIN family genes PgPIN2, PgPIN3 and PgPIN6 in P ginseng. PIN expression in ginseng adventitious root and culture seedling was analyzed with qRT-PCR technique. Results suggested that in ginseng adventitious root tip, PgPIN3 and PgPIN6 exhibited a high level of expression; in ginseng culture seedling root, PgPIN2 showed a high level of expression; in four-year-old Jilin ginseng at the fruit ripening stage, PgPIN2 and PgPIN6 were highly expressed in root and rhizome, while PgPIN3 had a high level in ginseng leaf,fruit and root. Tissue specific expression profile showed that PgPIN2 and PgP1N6 probably were involved in the development and tropism growth in ginseng roots, while PIN3 might be in relation to the growth and development of the aerial part of plants.