中文摘要：

利用与氨基选择性反应的荧光染料5一羧基荧光素琥珀酰亚胺酯（5～FAMSE）对豇豆花叶病毒（CPMV）表面的氨基进行修饰，制备了荧光功能化CPMV纳米粒（5-FAM：／CPMV）。对其结构形态和单、双光子荧光进行了测试，用毛细管电泳对其表面特性进行了考察，并将其用于Hela细胞的双光子荧光成像。研究表明，目标纳米粒粒径均匀，仍具有天然CPMV的特性，能进入Hela细胞，在波长800nm激光激发下可以成功地用于肿瘤细胞的成像分析，可望用于肿瘤靶向双光子荧光细胞成像方面的研究。

英文摘要：

The fluorescent nanoparticles（5-FAM/CPMV） based on cowpea mosaic virus（CPMV） has been prepared by modifying the amine groups on the surface of CPMV with amine-reactive fluorescent probe, 5-carboxyfluorescein succinimidyl ester（5-FAMSE）. The structure as well as one- and two-photon fluorescence of the nanoparticles have been investigated. The surface property of 5-FAM/CPMV has also been investigated by capillary electrophoresis. Finally, 5-FAM/CPMV was employed for the two-photon fluorescence（TPF） imaging of Hela cells. The results showed that the modification of CPMV had no obvious effect on the nanostructure morphology of CPMV, or the biospecific property of CPMV. 5-FAM/ CPMV could be taken up by Hela cell and the green fluorescence could be observed in cellsap using TPF microscopy with the excitation wavelength of 800 nm, indicating 5-FAM/CPMV has the potential in targeted cancer cell TPF imaging.