中文摘要：

利用^60 Co-γ射线辐射籼稻品种93-11,获得了两份卷叶突变体 rl3（t）-1和 rl3（t）-2.与野生型93-11相比,两个突变体叶片显著内卷,株高降低,穗长变短,并且结实率降低.细胞学分析表明,突变体叶片中泡状细胞数量的减少和形态的改变,可能是导致叶片卷曲的原因之一.遗传分析和等位性测验表明突变体受一对隐性核基因控制,且 rl3（t）-1和 rl3（t）-2等位.以卷叶突变体 rl3（t）-1与武运粳8号杂交的 F2分离群体作为定位群体,利用 SSR 标记和新发展的 STS 标记,将RL3（t）基因初步定位于第3染色体长臂上 STS 标记 M3-45和 SSR 标记 RM6676之间,遗传距离分别为5．5和4．4 cM.进一步扩大定位群体,结合新发展的 STS 分子标记,最终将该卷叶基因定位在 STS 标记 S3-39和 S3-36之间,该区段物理距离为46 kb.

英文摘要：

Two mutants with rolled leaves,derived from the indica cultivar 93-1 1 via the radiation of ^60 Co-γ ray in M2 generation,temporally designated as rl3 （t）-1 and rl3 （t）-2 ,were served as materials for exploring the mechanism underlying the rolling leaf characteristic.Morphological analysis showed that, these two mutants have typically adaxially rolled leaves.In addition,when compared with wild type 93-1 1 ,the plant heights and panicle lengths of rl3 （t）-1 and rl3 （t）-2 significantly decreased,as well as the seed setting percentage.Cytological analysis suggests that the rolled leaf phenotype may be caused by the change of number and size of bulliform cells.Genetic analysis indicated that rolled leaf character is controlled by a recessive nuclear gene.Crossing between the two mutants,the F1 [rl3 （t）-1/rl3 （t）-2 ]plants exhibit rolled leaf,suggesting they are allelic.To map the RL3 （t）gene,an F2 population was generated by crossing the rl3 （t）-1 mutants with Wuyunjing 8 as a mapping population.By using simple sequence repeat （SSR） markers and some new designed sequence tagged site （STS）markers,RL3 （t）was initially mapped in the region between the STS marker M3-45 and the SSR marker RM6676 with the genetic distances of 5 .5 cM and 4.4 cM, respectively,on the long arm of chromosome 3.Furthermore,with the enlarged population and more developed STS markers,RL3 （t）gene was finally delimited to a 46-kb long region governed by the STS markers S3-39 and S3-36.