为研究福建省杉木王种质资源的遗传多样性,在全面调查的基础上收集了福建省44份杉木王种质,并采用简单序列重复区间扩增多态性(ISSR)分子标记的方法分析其群体遗传多样性。结果表明,福建省杉木王主要分布在闽西、闽南和闽北地区,闽东地区分布较少。29条ISSR引物共扩增出237条带,平均每条引物扩增8.17条带;共有212条带具有多态性,多态性条带比率89%。福建省杉木王群体Nei's基因多样性指数为0.342 8,Shannon信息指数为0.502 6,显示出较高的遗传多样性。非加权类平均法聚类分析和遗传结构分析均显示,福建杉木王可划分为5个类群,其类群划分结果与样本间的地理位置分布较为一致。
On the basis of comprehensive survey,44 samples of the king of Chinese fir germplasm in Fujian provenances were collected,and the population genetic diversity was detected by inter-simple sequence repeats( ISSR) molecular markers. The results showed that the king of Chinese fir were mainly distributed in western,southern and northern of Fujian,but rarely distributed in eastern. Twenty-nine primers were amplified into 237 bands,8.17 bands on average from a primer amplification. Two hundred and twelve bands were polymorphic,accounting for 89%. The population Nei' s genetic diversity index was 0. 342 8,and the Shannon information index was 0.502 6,which revealed a relatively high genetic diversity. Both of the unweighted pair-group method with arithmetic means( UPGMA) clustering and genetic structure analysis showed the king of Chinese fir in Fujian provenances could be divided into 5 groups,which were comparatively agree with the geography distribution.