中文摘要：

以红色红曲菌Monascus ruber M7菌株为研究对象,对其核型进行分析,并将桔霉素与色素合成基因簇定位于染色体。通过原生质体制备,以脉冲场凝胶电泳进行核型分析。在此基础上,通过Southern杂交将桔霉素与色素合成基因簇定位于染色体。脉冲场凝胶电泳结果显示,红色红曲菌M7包含大小依次为4.9,4.3,3.7,3.4,3.0,2.3,2.1Mb的7条（I-VII）染色体,基因组大小约为23.7Mb。Southern杂交结果表明,桔霉素合成基因簇位于第IV条染色体上,色素合成基因簇位于第V条染色体上。

英文摘要：

Karyotype of Monascus ruber M7 strain was analyzed, and the biosynthetic gene clusters of citrinin and Monascus pigment（MP） were located on the chromosomes of the strain. Protoplasts of M. ruber M7 were prepared, and the karyotype of the strain was determined by pulsed-field gel electrophoresis（PFGE）. Biosynthetic gene clusters for citrinin and MP were localized on the chromosomes by Southern blotting. PFGE results showed that seven chromosomes were separated from the M. ruber M7 genome with the sizes of 4.9, 4.3, 3.7, 3.4, 3.0, 2.3 and 2.1Mb, respectively. Its genome size was thereby estimated at around 23.7Mb. Results of Southern blotting revealed that citrinin gene cluster was localized on the chromosome IV, while MP gene cluster was encoded by the chromosome V.