中文摘要：

目的：利用RP—HPLC—ELSD技术建立不同产地茯苓三萜的指纹图谱，为评价不同产地的茯苓药材的质量品质提供理论依据。方法：色谱柱为Kromasil100—5C18柱（4．6mm×250mm，5μm），流动相0．2％甲酸水溶液（A）-乙腈（B），采用梯度洗脱法，流速1．0mL·min^-1，柱温30℃，蒸发光检测条件：雾化温度40℃，压力350kPa，gain值为7；进样量20μL。结果：建立的指纹图谱中有18个共有峰，方法学考查表明，各主要色谱峰相对保留时间及峰面积的RSD均〈3％，峰面积占总峰面积〉10％的共有峰其相对峰面积差值均在±25％以内，符合指数图谱的技术要求，仪器的精密度良好，且供试品溶液至少12h内稳定。结论：该方法简便快速、准确可靠，可作为评价不同产地茯苓药材质量的有效手段。

英文摘要：

Objective： Investigation on RP-HPLC-ELSD fingerprint of triterpenes in Poria cocos from different origin place, in order to provide a theory evidence to evaluate the quality of P. cocos from different origin place, and improve the quality of P. cocos. Method： The chromatographic conditions were Kromasil 100-5 C18 column （4.6 mmx 250 mm, 5μm）, 0.2% methanoic acid （A） and acetonitrile （B） as gradient mobile phases, flow rate being 1.0 mL .min-1, column temperature at 30 ℃ , evaporative light-detection conditions： atomization temperature at 40 ℃, pressure of 350 kPa, gain value of 7, and 20 μL sample injection. Result： Fingerprint was established with 18 common peaks and methodology reached the required standard. Conclusion： The method is simple, rapid, accurate, and can be used as an effective means of P. cocos quality evaluation of different origin place.