中文摘要：

目的观察慢性不可预知应激（CUS）并配合孤养诱导模型大鼠抑郁样行为过程中神经递质水平的变化，探讨神经递质作为诊断抑郁症的客观、可量化标志物的可能性。方法利用CUS并配合孤养模型建立抑郁大鼠动物模型，以体质量、糖水偏爱率和旷场实验（穿格数和直立次数等）结果评定大鼠行为学的动态变化；采用高效液相-荧光法（HPLC-FD）测定血清中5-羟色胺（5-HT）、去甲肾上腺素（NE）、肾上腺素（E）、多巴胺（DA）、左旋多巴（L—DOPA）、5-羟基吲哚乙酸（5-HIAA）、高香草酸（HVA）、3，4-二羟基苯乙酸（DOPAC）、酪氨酸（Tyr）和色氨酸（Trp）等10种神经递质的含量；应用SPSS软件对体质量、糖水偏爱率和旷场数据等行为结果进行主成分分析，并采用回归分析方法分析行为学与神经递质变化间的相关性。结果①与正常对照组相比，造模期间第14天，模型组直立次数显著降低（P〈0．05）；造模期间第22天，模型组体质量、糖水偏爱率、穿格数和直立次数均显著降低（P〈0.05）。②与正常对照组相比，造模期间第6天，模型组Tyr和5-HIAA的含量显著降低（P〈0.05）；造模期间第9天，NE的含量显著降低（P〈0．05）；造模期间第22天，NE和5-HT的含量显著降低（P〈0．05）；造模期间第15和22天，NE，5-HIAA，HVA和5-HT的含量显著降低（P〈0．05）。③NE，HVA和5-HT的变化趋势为降低的程度逐渐增大，与行为学结果变化趋势接近；而Tyr的变化趋势为先升后降，5-HIAA，L-DOPA，DA，DOPAC，Trp和E呈波动性变化。主成分分析结果显示，第一主成分可代表93．47％的信息，结果可靠；相关性分析结果表明，5-HT，NE和HVA与第一主成分的相关性好。结论血清中神经递质在CUS诱导抑郁样行为过程中呈动态变化，其中NE，5-HT和HVA的变化可以在一定程度上反映大鼠的抑郁状态和程

英文摘要：

OBJECTIVE To observe the change of serum neurotransmitters in depression rats in- duced by chronic unpredictable stress （CUS） and to investigate the possibility of serum neurotransmit- ters being used a quantitative diagnostic biomarkers for depression. METHODS Depression was in- duced by CUS. Sucrose preference test and open-field test were applied to evaluate the behavior of CUS rats. The content of 5-hydroxytryptamine （5-HT）, norepinephrine （ NE）, epinephrine （ E）, dopamine （DA）, levodopa（L-DOPA）, 5-hydroxy-indole-3-acetic acid （5-HIAA）, homovanillic acid （HVA）, 3,4- dihydroxyphenylacetic acid （DOPAC）, tyrosine（Tyr） and tryptophan（Trp） in serum was determined by reverse phase high performance liquid chromatography with fluorescence detector. RESULTS ① Com- pared with normal control group, the body mass, preference sucrose, ambulation number and rearing number in CUS model rats showed a significant decrease after 21 d of stress exposure. ② Compared with normal control group, the Tyr and 5-HIAA levels was significantly lower in CUS model rats on the 6th day, NE level was significantly lower in CUS rats on the 9th day, NE and 5-HT levels was significantly lower in CUS rats on the 12th day, levels of NE, 5-HIAA, HVA and 5-HT were significantly lower in CUS rats on the 15th and the 22nd day.③NE, HVA and 5-HT showed a tendency of gradual decline, while Tyr dropped after rise. 5-HIAA, L-DOPA, DA, DOPAC, Trp and E showed fluctuations. The principal compoent analysis showed that the first principal component could represent 93.47% of the information. Correlation analysis showed that 5-HT, NE and HVA were closely related to the first principal compo nents. CONCLUSION During the development of rats＇ depression induced by CUS, the serum neuro- transmitters change significantly. NE, 5-HT and HVA are possibly the biomarkers for auxiliary diagnosis of depression.