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中文摘要：
为探明喀斯特地区丛枝菌根真菌（AMF）的遗传多样性特征,利用巢式PCR和DGGE相结合的分子生物学方法对茂兰喀斯特多个植被类型下的AMF遗传多样性进行了研究。结果表明,喀斯特地区AMF遗传多样性指数和物种丰富度分别平均为3.50和41,远高于非喀斯特对照样地的2.68和17,分析表明,喀斯特地区较高的AMF多样性与此地区丰富的植物多样性以及特殊的生态环境有关,是与喀斯特生态系统长期相互选择的结果。不同植被类型下的AMF多样性差异显著,相似性指数最高为0.34,喀斯特地区AMF的群落结构随着植被类型的改变发生显著变化;基因测序显示,喀斯特地区AMF的优势菌属是生态适应性很强的球囊霉属,在喀斯特石漠化生态恢复中具有较强的利用潜力。
英文摘要：
By the methods of nested PCR and DGGE,this paper studied the genetic diversity of arbuscular mycorrhizal fungi（AMF） under various vegetation types in Maolan Karst area,aimed to explore the AMF genetic diversity in Karst area.In the study area,the AMF genetic diversity index（3.50） and species abundance（41） were far higher than those（2.68 and 17,respectively） in non-Karst area.The high diversity of AMF in study area was closely related to the rich plant diversity and the special ecological environment,and was the result of long-term mutual choice of the AMF and Karst ecosystem.The AMF diversity under different vegetation types had significant difference,with the highest similarity index only 0.34.The community structure of the AMF changed significantly with vegetation type.Gene sequencing displayed that Glomus was the dominant genera,which had strong ecological adaptability and great potential in the ecological restoration of Karst rocky desertification.
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