中文摘要：

为探索板栗PPDK基因功能以及花粉直感效应分子机理,以板栗果实为材料,根据已登录的拟南芥、桃树、巨桉等多种其他植物物种的PPDK基因序列的保守区,设计特异性引物。利用RT-PCR技术克隆出板栗PPDK基因的编码区,并进行序列分析。结果表明,该基因长为3 293bp,包含一个完整的ORF,长2 676bp,编码892个氨基酸,分子量为97.89KDa。所编码的氨基酸序列与已知植物来源的若干PPDK基因相比,同源性大多在85%以上,证明所克隆的目的片段为PPDK基因,并将该序列登录到GenBank,登录号为KU234550。在PPDK基因所编码的氨基酸序列中发现了一个磷酸烯醇式丙酮酸利用酶磷酸化位点标记,一个拉链结构和多个磷酸化位点。同时推测出多肽链中有规则重复的构象,并同源建模PPDK蛋白整条肽链的三维空间结构。试验认为PPDK基因可能通过影响板栗光合作用进而使板栗在果实大小、淀粉含量等方面表现出明显的花粉直感效应。

英文摘要：

Through the test,a reference was provided for the studies on the function of pyruvate phosphate dikinase gene and metaxenia effect in Castanea mollissirna. Taking the fruit as materials, specific primers were designed on the basis of pyruvate phosphate dikinase （PPDK） gene from other plants in NCBI,PPDK gene was cloned and analyzed by RT-PCR techniques. A PPDK cDNA was cloned. The CDS length was 2676 bp,coding 892 amino acids, 97.8 KDa molecular weight. The sequence of PPDK had high similarity with other plants. GenBank accession number was KU234550. Besides, The PPDK contained some important motifs related with enzyme activities,such as Pyruvate phosphate dikinase,PEP/pyruvate binding domain,PEP-utilizing enzyme, mobile domain, a leucine zipper pattern and many phosphorylation sites. The secondary structure and tertiary structure were predicted. The studies suggested that chestnut showed obviously metaxenia effect on fruit size, starch content which may under the influence of PPDK on photosynthesis.