中文摘要：

采用电解法制备了粒径分布为（50±8）nm，表面呈正电性的纳米银粒子。基于该纳米银粒子，采用静电自组装技术在经过特殊处理、表面呈负电性的玻璃基底上生成了具有高效、稳定性的二维纳米结构银膜。该银膜外层纳米银粒子平均粒径约200nm，相邻纳米银粒子之间形成了不规则的纳米微区，平均尺寸达到200nm左右。基于该纳米银膜，采用便携式拉曼光谱仪对20个正常人血清样本进行了表面增强拉曼散射（SERS）光谱的检测。同时，也对同一正常人血清基于同种条件下制备的不同纳米银膜的SERS光谱进行了检测。实验发现：该纳米银膜对人血清的拉曼散射具有较好的增强效果，有望为人血清的研究提供新的途径。

英文摘要：

The fabrication of silver nanoarchitectures （Ag nanofilms） on glass substrates by using a low-cost electrochemical strategy at a proper voltage and polyvinyl alcohol concentration in electrolyte is reported. Ag nanofilms are grown and built with nanoblocks of two-dimensional spherelike nanostructures, which show a highly active and stable surface enhanced Raman scattering （SERS） effect. It shows that the average particle size of silver nanoparticles on the surface of the Ag nanofilms is about 200 nm, and the average size of the nanoblocks is also about 200 nm. The SERS spectra of the human sera for 20 healthy donors were studied on a portable Raman spectrometer, meanwhile, SERS spectra of the serum for the same person was also detected based on different Ag nanofilms prepared with the same conditions. The results show that the SERS effect of human serum is perfect based on the SERS substrate, which will provide a potential tool for analyzing human serum.